Genetic Engineering and Biotechnology

Polyethylene glycol (PEG) is a chemical agent used to facilitate the uptake of DNA into protoplasts (cells without walls) by inducing membrane fusion, which is a vector-free method of gene transfer.

Electroporation or electropermeabilization, is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell. In molecular biology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA.

• Several chemicals have been used to induce protoplast fusion.
• Sodium nitrate (NaN03), polyethene glycol (PEG), Calcium ions (Ca2+), Polyvinyl alcohol etc. are the most commonly used protoplast fusion inducing agents which are commonly known as chemical fusogens. 
• Hence, PEG is used for Fusing of protoplasts.
•  So, the correct answer is 'Fusing of protoplasts'.

Gene transfer technologies include techniques of electroporation, microinjection, macroinjection, biolistics or microprojectiles for DNA transfer (particle gun), liposome mediated gene transfer, calcium phosphate mediated DNA transfer, use of DAE-dextran to transfer gene, polycation-DMSO for DNA transfer, polyethylene glycol mediated transfection and gene transfer through peptides.

Microorganisms include bacteria, fungi, protozoa, some algae, viruses, viroids and also prions. Some other microorganisms are beneficial and contribute to human welfare. This post is about such useful microorganisms [useful microbes].

Red biotechnology(Bio-pharmaceutical) is a process that utilizes organisms to improve health care and help the body to fight diseases. It is a branch of modern biotechnology which is utilized in the field of medicine.

Many mechanisms are used to obtain the stem cells. The somatic cells or the reproductive cells can be induced to form the stem cells using genetic reprogramming method. Reprogramming can be performed using the factors or proteins which induce the normal body cells to be stimulated to form stem cells. Pluripotent stem cells are generally formed with the help of the induction mechanism. 

Biotechnology is the use of living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms or derivatives thereof, to make or modify products or processes for specific use. For thousands of years, humankind has used biotechnology in agriculture, food production, and medicine. 

Application of biotechnology occurs in agriculture, biomedicine and environmental field.

Biotechnology is the use of biological processes, organisms, or systems to manufacture products intended to improve the quality of human life. The earliest biotechnologists were farmers who developed improved species of plants and animals by cross pollination or cross breeding. 

The correct option is C

Genetic engineering encompasses techniques to modify the genome, including transgenic plant development, gene therapy, and vaccine research.

Modern biotechnology is based on the the methods of genetic engineering.  It is a technique which involves the introduction of foreign DNA carrying the desired gene of interest into the host organism & thus change the phenotype of the host organism.

Genetic engineering is the specific field concerned with the manipulation of an organism's genotype through the introduction of foreign genes.