Tag: biotechnology in agriculture

Questions Related to biotechnology in agriculture

Vector for T-DNA is

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Salmonella typhimurium

  2. Thermits aquaticus

  3. Agrobacterium tumefaciens

  4. Escherichia coli

  5. Bacillus thuringiensis

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Correct Option: C

Which of the following is correctly matched?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Agrobacterium tumefaciens- Tumour

  2. pBR 322- Enzyme

  3. Thermus aquaticus- Bt gene

  4. Ligase- Molecular scissors

  5. Hind II- Plasmid vector

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Correct Option: A

Pathogenic entity that is responsible for the production of tumour in most dicot plants is

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Retrovirus

  2. Bacteriophage

  3. Ti plasmid

  4. Vector

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Correct Option: C
Explanation:

Agrobacterium is a genus of Gram-negative bacteria that causes tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus. It contains "Ti" plasmid or tumor inducing plasmid, that causes  crown-gall disease in plants. The disease is characterised by a tumour-like growth or gall on the infected plant, often at the junction between the root and the shoot. Tumors are incited by the conjugative transfer of a DNA segment (T-DNA) from the bacterial tumour-inducing (Ti) plasmid to the plant cell naturally when the bacterium infects the plant through  a wound.


So, the correct answer is 'Ti plasmid'

Diseases like malaria and filariasis are transmitted through insect vectors .Vectors can be controlled by introducing fish

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Labeo

  2. Gambusia

  3. Hilsa

  4. Catla

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Correct Option: B

Consider the following statements:
1. AgrobacteriMm tMmejaciens is a soil bacterium.
2. AgrobacteriMm tumefaciens was the first vector used for introduction offoreign DNA in plant cells.
Which of the statements given above is/are correct?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. 1 only

  2. 2 only

  3. Both 1 and 2

  4. Neither 1 nor 2

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Correct Option: A

pBR $322$ which is frequently used as a vector for cloning gene in E.coli is?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. An original bacterial plasmid

  2. A modified bacterial plasmid

  3. A viral genome

  4. A transposon

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Correct Option: A

Which one is a cloning plasmid and not an expression plasmid?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. pBAD-18-Cam

  2. pBCSK

  3. pUC 18

  4. pET

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Correct Option: C

Second generation vaccines are prepared by recombinant DNA technology. Which out of the following are the examples of such vaccines?

botany biotechnology and its applications biotechnology in agriculture gm crops transgenic organisms

  1. Hepatitis B virus vaccine

  2. Herpes virus vaccine

  3. Salk's polio vaccine

  4. Both (A) and (B)

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Correct Option: D
Explanation:
Second generation vaccines are the vaccines which are prepared by recombinant DNA technique/ genetic engineering, e.g., hepatitis B virus vaccine, herpes virus vaccine and pneumonia vaccine. The yeast-derived recombinant hepatitis B virus vaccine is the first commercially available human vaccine produced by the genetic engineering technology. Hepatitis B vaccine is produced from transgenic yeast by recombinant DNA technology.
So, the correct answer is 'Both (A) and (B)'.

Golden rice is _____________________.

botany biotechnology and its applications biotechnology in agriculture gm crops transgenic organisms

  1. A variety of rice grown along the yellow river in China

  2. Long stored rice having yellow cocur tint

  3. A transgenic rice having gene for $\beta$-carotene

  4. Wild variety of rice with yellow colored grains

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Correct Option: C
Explanation:

Golden rice is a transgenic variety of rice (Oryza Sativa) which contains good quantities of $\beta$-carotene ( provitamin A- inactive state of vitamin A). $\beta$-carotene is a principle source of vitamin A. Since the grains (seeds) of the rice are yellow in colour due to $\beta$-carotene, the rice is commonly called golden rice. 

The transgenic plant 'Flavr Savr' tomato carries an artificial gene for

botany biotechnology and its applications biotechnology in agriculture gm crops transgenic organisms

  1. Delayed ripening process

  2. Longer shelf life

  3. Enhanced flavour

  4. All of these.

Show answer
Correct Option: D
Explanation:

Flavr Savr is a genetically modified tomato  for human consumption. The transgenic plant 'Flavr Savr' tomato carries an artificial gene for slowing down the ripening process of the tomato and thus prevent it from softening and retains its natural colour , flavour and its shelf life. 

So, the correct option is 'Option D' .