Tag: biotechnology in agriculture

Questions Related to biotechnology in agriculture

Insertional inactivation is related to

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Microinjection

  2. Gene gun

  3. Gel electrophoresis

  4. Selection of recombinations

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Correct Option: D
Explanation:

Insertional inactivation is the inactivation of a gene upon insertion of another gene inside in its place or within its coding sequence. This helps the selection of transformant and as well as recombinant colonies in selective antibiotic medium aided due to insertional inactivation of the antibiotic gene which becomes inactive due to foreign gene insert within its sequence. 

So, the correct option is Selection of recombination.

Hybridoma technology was developed by

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Taggart, 1982

  2. Vitella et al, 1982

  3. Prie and Saxton, 1987

  4. Milstein and Kohier, 1975

Show answer
Correct Option: D
Explanation:

Hybridoma technology is a technology of forming hybrid cell lines (called hybridomas).

A major event in r-DNA technology is _________________.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Separation

  2. Isolation

  3. Elution

  4. Both A and B

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Correct Option: D
Explanation:

r-DNA technology is the process involving the introduction of a foreign piece DNA containing a gene of interest into the genome of an organism. Isolation and separation is a step in the process in which various techniques are used to separate the required gene (containing the gene of interest) from the nucleus of the cell (free of the other cellular contaminants). The isolated DNA is then restriction digested and amplified to make several copies. It is then inserted into a vector for transfer into an appropriate host. Elution is a step involved in the process of separation of DNA. So, the correct answer is 'isolation and separation'.

In which of the following combinations, the compounds in ascending order based on their molecular weights are arranged

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. DNA, RNA, AMP, ADP, ATP

  2. DNA, RNA, ATP, ADP, AMP

  3. AMP, ADP, ATP, RNA, DNA

  4. AMP, ATP, ADP, DNA, RNA

Show answer
Correct Option: C
Explanation:

The arrangement of compounds in ascending order according to their molecular weight is AMP, ADP, ATP, RNA, DNA.

The most extensively used bacteria in genetic engineering is ____________.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Bacillus

  2. Clostridium

  3. Escherichia

  4. Salmonella

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Correct Option: C

Which of the following is not a method of introducing alien DNA into host cells?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Micro injection

  2. Electroporation 

  3. Being placed along with the cell into a gene gun

  4. Gel electrophoresis

Show answer
Correct Option: D
Explanation:
A. Microinjection is a technique in which DNA is directly injected into the host cells with the help of microinjection.
B. Electroporation is a technique in which pores are created in the cell membrane and thus, permeability is increased. As a result, foreign DNA can easily enter the cells.
C. Gene gun is a method to introduce alien DNA into host cells through microprojectile particles. The DNA coated gold or tungsten particles are bombarded with the host cells and alien DNA enters the cells.
D. Gel electrophoresis is a technique of separating DNA fragments on the basis of their size.
Hence, option D is not a method of introducing alien DNA into host cells.
So, the correct answer 'Gel electrophoresis'.

Identify the correct sequence of steps in the construction of recombinant DNA.
$(1)$ use restriction enzymes
$(2)$ use DNA ligase
$(3)$ remove plasmid from parent bacterium
$(4)$ introduce plasmid into new host bacterium.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. $1-2-3-4$

  2. $4-3-2-1$

  3. $3-1-2-4$

  4. $2-3-1-4$

Show answer
Correct Option: D
Explanation:
Generation  of rDNA steps:
1) The DNA for insulin is first disengaged. 
2) A plasmid made of DNA is expelled from a bacterial cell. 
3) A limitation protein cuts the plasmid DNA open, leaving sticky finishes. 
4) The insulin quality, with integral sticky closures, is included. 
5) DNA ligase catalyst grafts (joins) together with the plasmid DNA and the Insulin DNA. 
6) The plasmid (now hereditarily changed) is embedded again into the bacterium. 
7) The bacterium has cell, partitions and delivers duplicates of the plasmid. 
8) The Bacterium makes human insulin utilizing the quality in the plasmid. 
9) The insulin is removed from the bacterial culture.
So, the correct option '2−3−1−4'.

The first plasmid used for recombinant DNA technology is obtained from

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Bacillus thurinegenesis

  2. Thermus aquaticus

  3. Salmonella typhimurium

  4. E. Coli

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Correct Option: B

The process by which restriction endonuclease cut the DNA molecule?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Reduction

  2. Oxidation

  3. Hydrolysis

  4. Carboxylation

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Correct Option: A

In E.coli, a finished polypeptide has $162$ amino acids of which the first amino acid is not methionine compound. How many nucleotides of DNA are required to code this polypeptide?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. $486$

  2. $54$

  3. $489$

  4. $492$

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Correct Option: A