Tag: biotechnology in agriculture

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Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

  1. Reverse transcriptase-production of.cDNA from mRNA

  2. DNA polymerase-used in polymerase chain reaction to amplify section of DNA

  3. DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments

  4. Restriction enzyme-production of RFLPs
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Restriction enzymes cut DNA creating sticky ends of restriction fragments. While DNA ligase joins these sticky ends to form recombinant DNA. 

So, the correct option is 'DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments'. 

Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

  1. iii, iv

  2. i, iv

  3. i, ii

  4. ii, iii
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

In insertional inactivation, the Z-gene (encoding beta-galactosidase) is disrupted. If the gene is intact, the bacteria produce the enzyme and turn blue on chromogenic substrate (non-recombinant). If the DNA segment is inserted, the enzyme is not produced, and colonies remain white (recombinant).

Multiple choice botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of

  1. Silver or Tungsten

  2. Arsenic or Silver

  3. Gold or Tungsten

  4. None of these
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation
Biolistic method or gene gun method is a direct or vectorless method of introducing DNA into cells that involves bombardment of cells with high-velocity microprojectiles coated with DNA. In biolistic method, tungsten or gold particles, coated with foreign DNA are bombarded into target at a very high velocity.
So, the correct answer is 'Gold or tungsten'.
Multiple choice botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

Which of the following techniques can be used to introduce foreign DNA into the cell?

  1. Using disarmed pathogen

  2. Microinjection

  3. Gene gun

  4. All of these
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Vectors, gene gun ( biolistic ) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is All of these.

Multiple choice botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

Electroporation is

  1. Making transient pores in cell membranes to introduce gene constructs

  2. Fast passage of nutrients through phloem sieve pores by electric stimulation

  3. Opening of stomata by artificial light during night

  4. Purification of saline water with the help of membrane system
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Electroporation is a technique used in molecular biology to create temporary pores in cell membranes using an electrical pulse, allowing foreign DNA or other molecules to enter the cell.

Multiple choice botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

Genes can be inserted into human cells by

  1. PCR

  2. Xenotransplantation

  3. Modified viruses

  4. Modified microarrays
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Modified viruses (viral vectors) are commonly used in gene therapy to deliver therapeutic genes into human cells because of their natural ability to infect cells.

Multiple choice botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

Find the odd one among the following methods of rDNA transfer into host cell.

  1. Biolistics

  2. Gene gun

  3. Microinjection

  4. Retroviral vector
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

There are various methods of making the host cell competent for transformation with Recombinant DNA technology.

Microinjection method is one where the recommended DNA is directly injected into the nucleus of the animal cell. In the gene gun method, the bombardments on plant cell with high-velocity microparticles of gold or tungsten coated with DNA is done which is known as biolistic or gene gun.
The pathogen vectors are allowed to infect the cell and transfer the recombinant DNA into the host cell which is known as the retroviral vector method.
So, the correct option is 'Biolistics'.