Tag: biotechnology in agriculture

Questions Related to biotechnology in agriculture

When E coli with DNA $^{15}N ^{15}N $ is allowed to grow in a medium containing $^{14}N$ the number of DNA strands with $^{15}N$ after five generations is: 

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. 1

  2. 2

  3. 8

  4. 16

  5. 30

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Correct Option: A

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Reverse transcriptase-production of.cDNA from mRNA

  2. DNA polymerase-used in polymerase chain reaction to amplify section of DNA

  3. DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments

  4. Restriction enzyme-production of RFLPs

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Correct Option: C
Explanation:

Restriction enzymes cut DNA creating sticky ends of restriction fragments. While DNA ligase joins these sticky ends to form recombinant DNA. 

So, the correct option is 'DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments'. 

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. iii, iv

  2. i, iv

  3. i, ii

  4. ii, iii

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Correct Option: D

In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Silver or Tungsten

  2. Arsenic or Silver

  3. Gold or Tungsten

  4. None of these

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Correct Option: C
Explanation:
Biolistic method or gene gun method is a direct or vectorless method of introducing DNA into cells that involves bombardment of cells with high-velocity microprojectiles coated with DNA. In biolistic method, tungsten or gold particles, coated with foreign DNA are bombarded into target at a very high velocity.
So, the correct answer is 'Gold or tungsten'.

Which of the following techniques can be used to introduce foreign DNA into the cell?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Using disarmed pathogen

  2. Microinjection

  3. Gene gun

  4. All of these

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Correct Option: D
Explanation:

Vectors, gene gun ( biolistic ) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is All of these.

Electroporation is

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Making transient pores in cell membranes to introduce gene constructs

  2. Fast passage of nutrients through phloem sieve pores by electric stimulation

  3. Opening of stomata by artificial light during night

  4. Purification of saline water with the help of membrane system

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Correct Option: A

Genes can be inserted into human cells by

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. PCR

  2. Xenotransplantation

  3. Modified viruses

  4. Modified microarrays

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Correct Option: C

Find the odd one among the following methods of rDNA transfer into host cell.

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Biolistics

  2. Gene gun

  3. Microinjection

  4. Retroviral vector

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Correct Option: A
Explanation:

There are various methods of making the host cell competent for transformation with Recombinant DNA technology.

Microinjection method is one where the recommended DNA is directly injected into the nucleus of the animal cell. In the gene gun method, the bombardments on plant cell with high-velocity microparticles of gold or tungsten coated with DNA is done which is known as biolistic or gene gun.
The pathogen vectors are allowed to infect the cell and transfer the recombinant DNA into the host cell which is known as the retroviral vector method.
So, the correct option is 'Biolistics'.

Gene transfer is present in:-

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Biolistics

  2. Hybridization

  3. Tissue culture

  4. Vegetative propagation

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Correct Option: A

Which of the following is not a method of gene transfer in Bacteria?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Transformation

  2. Transduction

  3. Conjugation

  4. Gametangia contact

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Correct Option: A