Tag: biotechnology in agriculture

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Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Which statement is wrong?

  1. Nucleic acid is fragmented by nucleases

  2. Construction of recombinant DNA involves cleaving DNA segments with endonuclease and rejoining with ligase.

  3. Genetic engineering is making artificial limbs and diagnostic instruments
  4. $T _1$ plasmid transforms cells of plants.
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

In plant genetic engineering, the Ti plasmid can be used to carry foreign genes into plant cells. The Ti plasmid is the disease-causing agent of the soil-borne bacteria Agrobacterium tumefaciens. The Ti plasmid was then used to transform petunia cells in culture. So, the correct option is "D" (T1 plasmid transforms cells of plants).

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Ti-plasmid used in genetic engineering has been modified by

  1. Adding tumour forming genes

  2. Deleting tumour forming genes

  3. Adding genes for endonucleases

  4. Deleting genes for endonucleases
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
The correct option is B.
Ti plasmid that is tumer-inducing plasmid refers to the plasmid of Agrobacterium tumefaciens. Role of Ti plasmid in biotechnology is that it can be modified into a cloning vector by removing the genes responsible for pathogenicity.
Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Plasmids are suitable vectors for gene cloning because they are

  1. Small circular DNA molecules with their own origin of replication site

  2. Small circular DNA molecules which can integrate with host chromosomal DNA

  3. Having antibiotic genes

  4. Able to shuttle between prokaryotic and eukaryotic cells
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Plasmids are ideal vectors because they are small, circular, and contain an origin of replication, which ensures they can be maintained and copied by the host cell.

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Plasmids are used as vectors in genetic engineering because of their

  1. Resistance to antibiotics

  2. Resistance to restriction enzymes

  3. Ability to carry foreign genes

  4. Ability to cause infection in host
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Plasmids are used as vectors because they can be engineered to carry foreign genes and replicate within a host organism.

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Insect tolerant gene from Bacillus thuringiensis is introduced using $T 1$ plasmid of _______.

  1. Escherichia coli

  2. Agrobacterium tumefaciens

  3. Haemophilus influenzae

  4. Arabidopsis thaliana
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The Ti plasmid of Agrobacterium tumefaciens is the standard tool used to introduce insect-resistant genes, such as those from Bacillus thuringiensis, into plants.