Tag: biotechnology in agriculture

Questions Related to biotechnology in agriculture

Tumour inducing plasmid used in producing transgenic plants is that of ________________.

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Escherichia coli

  2. Bacillus thuringiensis

  3. Agrobacterium tumefaciens

  4. Staphylococcus aureus

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Correct Option: C

Which statement is wrong?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Nucleic acid is fragmented by nucleases

  2. Construction of recombinant DNA involves cleaving DNA segments with endonuclease and rejoining with ligase.

  3. Genetic engineering is making artificial limbs and diagnostic instruments

  4. $T _1$ plasmid transforms cells of plants.

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Correct Option: D
Explanation:

In plant genetic engineering, the Ti plasmid can be used to carry foreign genes into plant cells. The Ti plasmid is the disease-causing agent of the soil-borne bacteria Agrobacterium tumefaciens. The Ti plasmid was then used to transform petunia cells in culture. So, the correct option is "D" (T1 plasmid transforms cells of plants).

Ti-plasmid used in genetic engineering has been modified by

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Adding tumour forming genes

  2. Deleting tumour forming genes

  3. Adding genes for endonucleases

  4. Deleting genes for endonucleases

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Correct Option: B
Explanation:
The correct option is B.
Ti plasmid that is tumer-inducing plasmid refers to the plasmid of Agrobacterium tumefaciens. Role of Ti plasmid in biotechnology is that it can be modified into a cloning vector by removing the genes responsible for pathogenicity.

T$ _i$ plasmid transforms cells of

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Animals

  2. Plants

  3. Bacteria

  4. Fungi

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Correct Option: B

In genetic engineering which is used for transfer of genes from one cell to another

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Vector

  2. Probe

  3. Plasmid

  4. Virus

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Correct Option: A

Natural genetic engineer is

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Pseudomonas putida

  2. Agrobacterium tumefaciens

  3. Escherichia coli

  4. Bacillus sublilis

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Correct Option: B

$T _1$ plasmid is used for making transgenic plants. It is obtained from

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Azotobacter

  2. Agrobacterium tumefaciens

  3. Rhizobium in leguminous root

  4. Yeast

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Correct Option: B

Plasmids are suitable vectors for gene cloning because they are

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Small circular DNA molecules with their own origin of replication site

  2. Small circular DNA molecules which can integrate with host chromosomal DNA

  3. Having antibiotic genes

  4. Able to shuttle between prokaryotic and eukaryotic cells

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Correct Option: A

Plasmids are used as vectors in genetic engineering because of their

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Resistance to antibiotics

  2. Resistance to restriction enzymes

  3. Ability to carry foreign genes

  4. Ability to cause infection in host

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Correct Option: C

Insect tolerant gene from Bacillus thuringiensis is introduced using $T 1$ plasmid of _______.

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Escherichia coli

  2. Agrobacterium tumefaciens

  3. Haemophilus influenzae

  4. Arabidopsis thaliana

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Correct Option: B