Tag: insertion and ligation of dna
Questions Related to insertion and ligation of dna
The term 'recombinant DNA' refers to
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DNA of the host cell
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DNA with a piece of foreign DNA
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DNA with selectable marker
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DNA with more than one recognition sites
The term 'chimeric DNA' refers to
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DNA with overhanging stretches
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DNA with palindromic sequence
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A recombinant DNA
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Molecular scissors
Precipitates of purified DNA after the addition of chilled ethanol can be seen as a collection of fine threads in suspension. This process is referred as _____________.
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DNA transformation
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DNA ligation
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DNA spooling
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DNA duplication
The purified DNA, after treatment with various enzymes, precipitates out after addition of chilled ethanol. This is viewed as a collection of fine threads in the suspension, and is easily collected. The process is known as DNA spooling.
You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. which of the following strategies is most likely to be successful?
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Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into a plasmid
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Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into bacteria. Screen colonies for for the ability to glow in the dark.
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Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark.
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Isolate mRNA from the organism, reverse transcribe and genetate cDNA, Insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark.
Insertional inactivation is related to
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Microinjection
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Gene gun
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Gel electrophoresis
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Selection of recombinations
Insertional inactivation is the inactivation of a gene upon insertion of another gene inside in its place or within its coding sequence. This helps the selection of transformant and as well as recombinant colonies in selective antibiotic medium aided due to insertional inactivation of the antibiotic gene which becomes inactive due to foreign gene insert within its sequence.
Hybridoma technology was developed by
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Taggart, 1982
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Vitella et al, 1982
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Prie and Saxton, 1987
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Milstein and Kohier, 1975
Hybridoma technology is a technology of forming hybrid cell lines (called hybridomas).
A major event in r-DNA technology is _________________.
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Separation
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Isolation
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Elution
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Both A and B
r-DNA technology is the process involving the introduction of a foreign piece DNA containing a gene of interest into the genome of an organism. Isolation and separation is a step in the process in which various techniques are used to separate the required gene (containing the gene of interest) from the nucleus of the cell (free of the other cellular contaminants). The isolated DNA is then restriction digested and amplified to make several copies. It is then inserted into a vector for transfer into an appropriate host. Elution is a step involved in the process of separation of DNA. So, the correct answer is 'isolation and separation'.
In which of the following combinations, the compounds in ascending order based on their molecular weights are arranged
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DNA, RNA, AMP, ADP, ATP
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DNA, RNA, ATP, ADP, AMP
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AMP, ADP, ATP, RNA, DNA
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AMP, ATP, ADP, DNA, RNA
The arrangement of compounds in ascending order according to their molecular weight is AMP, ADP, ATP, RNA, DNA.
The most extensively used bacteria in genetic engineering is ____________.
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Bacillus
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Clostridium
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Escherichia
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Salmonella
Which of the following is not a method of introducing alien DNA into host cells?
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Micro injection
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Electroporation
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Being placed along with the cell into a gene gun
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Gel electrophoresis