Tag: cutting, insertion and ligation of dna

Quizzes (0) Questions (116)

Questions Related to cutting, insertion and ligation of dna

Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

Elution means

  1. Separation of DNA fragments on agarose gel

  2. Cutting and extraction of DNA bands from the agarose gel

  3. Making the DNA bands visible under UV radiation

  4. Isolation of alien DNA from the choice organism
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Elution is the process of extracting DNA fragments from an agarose gel after they have been separated.

Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

A dicotyledonous plant forms crown gall when

  1. Agrobacterium tumefaciens comes in contact with the plant

  2. Agrobacterium rhizogenes comes in contact with the plant

  3. A specific part of DNA from the Ti plasmid gets integrated with the plant chromosome

  4. A specific part of DNA from the Ri plasmid gets integrated with the plant chromosome
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Crown gall disease is caused by Agrobacterium tumefaciens, which transfers a specific segment of its Ti plasmid (T-DNA) into the plant genome.

Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

Find the correct statement with respect to function or properties of RNA polymerase.

  1. Bacteria have $3$ different types of RNA polymerase

  2. RNA polymerase binds to promoter and initiate transcription

  3. It uses nucleoside monophosphate as substrate

  4. RNA polymerase polymerizes in a template independent manner
Reveal answer Fill a bubble to check yourself
A Correct answer
Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

  1. $1, 3, 2, 4, 5$
  2. $3, 2, 1, 5, 4$
  3. $1, 3, 5, 4, 2$
  4. $5, 1, 3, 4, 2$
Reveal answer Fill a bubble to check yourself
A Correct answer
Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

  1. Reverse transcriptase-production of.cDNA from mRNA

  2. DNA polymerase-used in polymerase chain reaction to amplify section of DNA

  3. DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments

  4. Restriction enzyme-production of RFLPs
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Restriction enzymes cut DNA creating sticky ends of restriction fragments. While DNA ligase joins these sticky ends to form recombinant DNA. 

So, the correct option is 'DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments'. 

Multiple choice botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

  1. iii, iv

  2. i, iv

  3. i, ii

  4. ii, iii
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

In insertional inactivation, the Z-gene (encoding beta-galactosidase) is disrupted. If the gene is intact, the bacteria produce the enzyme and turn blue on chromogenic substrate (non-recombinant). If the DNA segment is inserted, the enzyme is not produced, and colonies remain white (recombinant).

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Enzyme' Taq polymerase' used in PCR, has been isolated from bacterium _________________.

  1. Agrobacterium tumefaciens

  2. Thermus aquaticus

  3. Streptomyces albus

  4. Escherichia coli
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The final step of PCR is the extension, wherein Taq DNA polymerase is isolated from a thermophilic bacterium Thermus aquaticus.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

The polymerase chain reaction is a technique used for ____________.

  1. Amplification of DNA

  2. Amplification of enzymes

  3. Amplification of proteins

  4. All of these
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Polymerase chain reaction is a technique used for amplification of DNA fragments. Its amplification cycle involve three steps - denaturation, annealing and polymerisation which are repeated for 'n', cycles.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following statements are correct for the enzyme Taq polymerase?


(i) It remains active during the high temperature-induced denaturation of dsDNA.

(ii) It requires primers for carrying out the process of polymerisation.
(iii) It synthesizes the RNA region between the primers, using $dNTPs$ and $Mg^{2+}$ _______________.

  1. (i) and (ii)

  2. (ii) and (iii)

  3. (i), (ii) and (iii)

  4. None of these
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The final step of PCR is the extension, wherein Taq DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesizes the DNA region between the primers, using dNTPs (deoxynucleoside triphosphates) and $Mg^{2+}$. The primers are extended towards each other so that the DNA segment lying between the two primers is copied. The optimum temperature for this polymerisation step is $72^o$$C$. Taq polymerase remains active during high temperature-induced denaturation of double-stranded DNA.