Tag: cutting, insertion and ligation of dna

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Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

One of the major advantages of transforming bacteria with a plasmid that contains DNA of interest is to amplify the DNA.
What other laboratory technique can be used to achieve the same result?

  1. Polymerase chain reaction

  2. Restriction fragment length polymorphism analysis

  3. DNA hybridization

  4. Gel electrophoresis
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Polymerase chain reaction is a method of amplifying a specific segment of DNA by making multiple copies of it, using primers and DNA polymerase

So, the correct answer is 'Polymerase chain reaction'

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction is used for

  1. In vivo replication

  2. In vivo synthesis of mRNA

  3. In vitro replication of DNA segment

  4. In vitro synthesis of mRNA
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

PCR is an in vitro technique used to replicate specific DNA segments outside of a living organism.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

PCR and RFLP are employed in

  1. DNA sequencing

  2. Genetic engineering

  3. Study of enzymes

  4. Genetic transformation
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

PCR and RFLP (Restriction Fragment Length Polymorphism) are fundamental tools used in genetic engineering for DNA analysis, cloning, and mapping.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

An analysis of chromosomal DNA using Southern hybridisation technique does not use

  1. Blotting

  2. Autoradiography

  3. PCR

  4. Electrophoresis
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Southern hybridization involves DNA digestion, electrophoresis, blotting, and probe hybridization. PCR is a separate technique for amplification and is not a standard step in the Southern blotting procedure itself.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which ones are simple sequence repeats (SSRs)

  1. Minisatellite sequences

  2. Microsatellites

  3. Pseudogenes

  4. Multiple gene family.
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Simple sequence repeats (SSRs), sometimes described as genetic 'stutters,' are DNA tracts in which a short base-pair motif is repeated several to many times in tandem (e.g. CAGCAGCAG). These sequences experience frequent mutations that alter the number of repeats similar to Multigene Family, a set of genes descended by duplication and variation from some ancestral gene.

So, the correct answer is 'Multiple gene family.'

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction employs

  1. Primers and DNA ligase

  2. DNA ligase only

  3. DNA polymerase only

  4. Primers and DNA polymerase
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

PCR requires primers to define the target region and a DNA polymerase to extend the strands. DNA ligase is not used in PCR.