Tag: cutting, insertion and ligation of dna

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Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following can't be achieved using PCR ?

  1. Detect HIV in AIDS suspect

  2. detect mutations in cancer patients

  3. Detect antigen - antibody interaction

  4. Detect specific micro organism from soil
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

PCR detects genetic material (DNA/RNA). Antigen-antibody interactions are detected using techniques like ELISA, not PCR.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

First technique and product produced by biotechnology are:

  1. PCR and detection of disease

  2. ELISA and detection of AIDS

  3. PCR and medicines

  4. Fermentation and Ethanol
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

While fermentation is an ancient biotechnological process, in the context of modern molecular biotechnology, PCR and its application in disease detection are often cited as foundational developments.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

  1. $5$' ______ GAATTC ________$3$

    $3$' _______ CTTAAG __________$5$'
  2. $5$' __________CACGTA _________ $3$'

    $3$'________ CTCAGT ________ $5$'
  3. $5$' ________ CGTTCG ________ $3$'

    $3$' _________ATGGTA _________$5$'
  4. $5$' _________ GATATG _________$3$'

    $3$'________CTACTA ________$5$'
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The sequence 5'-GAATTC-3' paired with 3'-CTTAAG-5' is the classic palindromic recognition site for the restriction enzyme EcoRI, which cuts between G and A.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following technique is employed to check the progression of a restriction enzyme digestion?

  1. PCR

  2. Bioreactor

  3. Gel electrophoresis

  4. Micro injection
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Answer is option C i.e. "Gel electrophoresis"
Gel electrophoresis is the technique which can check DNA fragment progression. Restriction enzyme digest DNA into fragments. Each fragment has different size. When gel electrophoresis is performed each fragment with different sizes are separated on the gel.
While, PCR is used for DNA amplification. Bioreactors are the fermentors used for fermentation process and micro-injection is a method used to transfer genes between animals.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following is not required in PCR?

  1. DNA primer

  2. DNA template

  3. RNA primer

  4. Taq polymerase
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

DNA primer is not required for a PCR reaction. 

The reason for RNA primers to be used in PCR is the non availability of DNA primers. The RNA primers complimentary to the cellular DNA are easily synthesized by the DNA Primase enzyme which is nothing but RNA polymerase just like mRNA.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

The areas of application of PCR include:


A. Production of monoclonal antibodies.
B. Insertion of recombinant DNA into an organism.
C. Diagnosis of a specific mutation.
D. Detection of plant pathogens.

  1. A B

  2. B, C

  3. C, D

  4. A, B, C, D
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Answer is option C i.e. "C, D"

PCR is used for diagnosis of diseases in animals and plants, it is also used to identify mutations in DNA. Major applications of PCR are:
(1) Clinical Diagnosis
(2) DNA Sequencing
(3)  Gene Manipulation and Expression Studies
(4) Forensic Medicine and (5) Gene Cloning.

While, Production of monoclonal antibodies is done by Hybridoma technology and insertion of recombinant DNA into organisms is done by transformation or transduction.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following are all Nucleotides?

  1. Adenosine, Cytidylic acid, Cytosine

  2. Adenylic acid, Cytidylic acid, Guanylic acid

  3. Cytidine, Adenine, Adenylic acid

  4. Uracil, Thymidine, Thymidylic acid
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
  • Nucleotides are compound consisting of a nucleoside linked to a phosphate group. Nucleotides form the basic structural unit of nucleic acids such as DNA and RNA. For example, Adenylic acid, Guanylic acid, Thymidylic acid, and Cytidilic acid and 5'uridylic acid.
  • Hence Adenylic acid, Cytidilic acid, Guanylic acid are all Nucleotides.
  • So, the correct answer is 'Adenylic acid, Cytidilic acid, Guanylic acid'.
Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Kary B Mullis got Nobel prize for discovering 

  1. DNA polymerase enzyme

  2. Polymerase chain reaction

  3. Restriction endonucleases

  4. Western blotting
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

DNA polymerase enzyme synthesizes DNA strands by adding deoxyribonucleotides to 3' end of the primer via phosphodiester bonds; it uses parental DNA as a template. Kary Mullis got Nobel prize, in 1993 in chemistry, for the invention of polymerase chain reaction (PCR).  PCR - technique amplify the gene of interest.