Tag: cutting, insertion and ligation of dna

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Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

DNA is amplified by __________________.

  1. PCR technique

  2. Southern Blotting technique

  3. Northern Blotting technique

  4. Nucleic acid hybridization technique
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

PCR (Polymerase Chain Reaction) is the standard laboratory technique used to amplify specific segments of DNA.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which one of the following enzymes is used in PCR reaction?

  1. Taq polymerase

  2. RNA polymerase

  3. Rib nuclease

  4. End nuclease
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Taq polymerase is the thermostable enzyme essential for the extension phase of the PCR reaction, allowing it to withstand high temperatures.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction technique is used for.

  1. DNA amplification

  2. Screening gene libraries

  3. Synthesis of polymerase

  4. DNA sequencing
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The primary purpose of the Polymerase Chain Reaction (PCR) is to create millions of copies of a specific DNA sequence, known as DNA amplification.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

If the initial number of template DNA molecules in a PCR reaction is $1000$, the number of product DNA molecules at the end of $20$ cycles will be closest to.

  1. $10^3$
  2. $10^6$
  3. $10^9$
  4. $10^{12}$
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

The number of DNA molecules after n cycles is calculated as N = N0 * 2^n. Here, N0 = 1000 and n = 20. So, N = 1000 * 2^20. Since 2^10 is approximately 10^3, 2^20 is approximately 10^6. Thus, 1000 * 10^6 = 10^9.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which does not describe a function of the DNA polymerase molecule?

  1. Recognise the free nucleotide that pairs with the base on the template strand of DNA

  2. Read the strand of template DNA and recognise the base there

  3. Proofread to ensure that the proper base has been incorporated

  4. Make the proper nucleotide to match with the base read on the template strand
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

DNA polymerase is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building blocks of DNA. The function of DNA polymerase is to replicate, proofread and repair DNA.DNA polymerases add nucleotide bases only when an RNA primer, a short piece of RNA, is already present. 

So, the correct option is 'Make the proper nucleotide to match with the base read on the template strand'.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

To carry out a polymerase chain reaction (PCR), one must have the catalytic DNA polymerase and

  1. a blueprint or gene map of the sequence one wish to copy

  2. a number of primers from either side of the target DNA in order to get the polymerase replication process- going

  3. a DNA synthesizer machine

  4. a DNA probe
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Polymerase Chain Reaction is a method widely used to make many copies of a specific DNA segment. Using PCR, a single (or more) copy of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment. Components of PCR include DNA template, DNA polymerase, two DNA primers, nucleotides.

So, the correct option is 'a number of primers from either side of the target DNA in order to get the polymerase replication process-ongoing'.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

What is the function of the polymerase chain reaction in genetic engineering?

  1. Cut DNA into many fragments

  2. Carry DNA into a new cell

  3. Link together newly joined fragments of DNA

  4. Make millions of copies of a specific segment of DNA
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Polymerase Chain Reaction is a method widely used to make many copies of a specific DNA segment. Using PCR, a single (or more) copy of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.

So, the correct option is 'Make millions of copies of a specific segment of DNA'.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

In the $PCR$ technology the $DNA$ segment is replicated over a billion times. This repeated replication is catalyzed by the enzyme.

  1. $DNA$ polymerase

  2. Taq polymerase
  3. $DNA$ dependent $RNA$ polymerase

  4. Primase
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq Pol or simply Taq. It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA.

T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75–80 °C, with a half-life of greater than 2 hours at 92.5 °C, 40 minutes at 95 °C and 9 minutes at 97.5 °C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72 °C.

So the correct option is 'Taq polymerase'.