Tag: gene cloning procedure
Questions Related to gene cloning procedure
Which of the following is required for microinjection method of gene transfer?
The term "competent" refers to
Micro-injection is a method used to
If a person obtains transformants by inserting a recombinant DNA within the coding sequence of enzyme -galactosidase, he will separate out recombinants from non-recombinants by which of the following observations?
In the process of insertional inactivation ________________.
If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the untransformed recipient cells will die. The product produced by transformant bacteria that digests the ampicillin antibiotic is called as -
The correct sequence of making a cell competent is
Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement 2: Presence of insert results into insertional inactivation of -galactosidase enzyme and the colonies do not produce any colour.
rDNA is
Direct visual selection method for the selection of recombinant host cells on the basis of their inability to produce colour in the presence of chromogenic substrate can be applied if the vector used is a