Tag: gene cloning procedure

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Questions Related to gene cloning procedure

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

The whole cycle of gene amplification requires

  1. 15 minutes

  2. 30 minutes

  3. 3 hours

  4. 24 hours.
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The denaturation stage of PCR takes about 1–2 minutes. The thermocycler then lowers the temperature to about 50° to 60°C, which allows the short, oligonucleotide primers to anneal to their complementary sequences on the single-stranded DNA molecules. The annealing stage of PCR lasts about 30 seconds. Hence, the whole cycle of gene amplification requires 30 minutes.

So, the correct answer is '30 minutes.'

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Enzyme required for polymerase chain reaction (PCR) is

  1. RNA polymerase

  2. Ribonuclease

  3. Taq polymerase

  4. Endonuclease
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

PCR requires a thermostable DNA polymerase to synthesize new DNA strands during the extension phase. Taq polymerase, isolated from Thermus aquaticus, is the standard enzyme used because it withstands high temperatures.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Thermal cycle is used in

  1. Radioactivation

  2. Chemical reaction

  3. Polymerase chain reaction

  4. Enzyme catalysed reactions
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

A thermal cycler is an instrument used to perform PCR by precisely controlling the temperature changes required for denaturation, annealing, and extension.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

One of the methods of which DNA cannot be transferred to the host cell is by

  1. Microinjection

  2. Gene gun

  3. Disarmed pathogen vectors

  4. Polymerase chain reaction
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

PCR is a technique for amplifying DNA in vitro. It is not a method for transferring DNA into a host cell; methods like microinjection, gene guns, and vectors are used for transformation.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction

  1. Is a method of synthesising human protein from human DNA

  2. Uses restriction enzymes

  3. Can produce billions of copies of a DNA fragment

  4. Takes place naturally in bacteria
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

PCR is specifically designed to amplify a specific DNA sequence, producing billions of copies from a small starting amount of template DNA.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

During PCR technique the pairing of primers to ssDNA segment is called

  1. Denaturation

  2. Annealing

  3. Polymerisation

  4. Isolation
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
PCR - technique amplify the DNA sample in three steps namely denaturation of target DNA, annealing and polymerization. Denaturation refers to the thermal cycle that separates the DNA strands and forms the ssDNA. Annealing refers to the pairing of primers to the ssDNA and polymerisation is the extension of primer into complete DNA strand complementary to the template strand. 
So, the correct answer is 'Annealing'
Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

How many copies of the DNA sample are produced in PCR technique after 6-cycle?

  1. $4$
  2. $32$
  3. $64$
  4. $16$
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Polymerase chain reaction (PCR) is a technique that exponentially amplifies a single copy of a specific DNA segment. It generates thousands of copies of that specific DNA segment. After completion of one cycle, 2 copies are produced from a single DNA segment. After completion of the second cycle, 2$^2$ = 4 copies are produced. Similarly, after n$^{th}$ cycle, 2$^2$ copies are produced, where n is the number of cycles. Hence, after completion of 6 cycle, 2$^6$ = 64 copies will be produced. 

Thus, the correct answer is '64.'

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following is not necessary to execute polymerase chain reaction successfully?

  1. All four DNA bases

  2. Short DNA base pairs

  3. DNA polymerase

  4. DNA library
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Polymerase Chain Reaction (PCR) is widely used the method in genetic engineering to prepare the multiple copies of desired DNA. It includes steps of denaturation, annealing extension and last amplification. As DNA is the polymer of four DNA bases present in the base pair and polymerase chain reaction is done in the presence of DNA polymerase enzyme of the DNA that re-studies by the researchers which is said to be DNA library so it is not required in the reaction.

So the correct option is 'DNA library'.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

In reference to DNA polymerase III, which statement is wrong?

  1. It requires ATP For polymerase action.

  2. Required for PCR

  3. More active than DNA Pol I & II

  4. Requires a previously made template to work on
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. The DNA polymerase typically used in PCR is called Taq polymerase, after the heat-tolerant bacterium from which it was isolated (Thermus aquaticus).
So, the correct option is 'Required for PCR'.
Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerisation of DNA is in

  1. $3' \rightarrow 5'$ direction
  2. $5' \rightarrow 3'$ direction
  3. Both $3' \rightarrow 5'$ and $5' \rightarrow 3'$ direction

  4. None of the above
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
DNA polymerase has 5' $\rightarrow$ 3' activity. All known DNA replication systems require a free 3' hydroxyl group before synthesis can be initiated (note: the DNA temple is read in 3' to 5' direction whereas a new strand is synthesized in 5' to 3' direction).
So, the correct answer is "5' $\rightarrow$ 3' ".