Tag: gene cloning procedure
Questions Related to gene cloning procedure
The whole cycle of gene amplification requires
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15 minutes
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30 minutes
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3 hours
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24 hours.
The denaturation stage of PCR takes about 1–2 minutes. The thermocycler then lowers the temperature to about 50° to 60°C, which allows the short, oligonucleotide primers to anneal to their complementary sequences on the single-stranded DNA molecules. The annealing stage of PCR lasts about 30 seconds. Hence, the whole cycle of gene amplification requires 30 minutes.
Enzyme required for polymerase chain reaction (PCR) is
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RNA polymerase
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Ribonuclease
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Taq polymerase
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Endonuclease
Thermal cycle is used in
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Radioactivation
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Chemical reaction
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Polymerase chain reaction
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Enzyme catalysed reactions
One of the methods of which DNA cannot be transferred to the host cell is by
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Microinjection
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Gene gun
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Disarmed pathogen vectors
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Polymerase chain reaction
Polymerase chain reaction
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Is a method of synthesising human protein from human DNA
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Uses restriction enzymes
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Can produce billions of copies of a DNA fragment
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Takes place naturally in bacteria
During PCR technique the pairing of primers to ssDNA segment is called
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Denaturation
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Annealing
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Polymerisation
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Isolation
How many copies of the DNA sample are produced in PCR technique after 6-cycle?
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$4$
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$32$
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$64$
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$16$
Polymerase chain reaction (PCR) is a technique that exponentially amplifies a single copy of a specific DNA segment. It generates thousands of copies of that specific DNA segment. After completion of one cycle, 2 copies are produced from a single DNA segment. After completion of the second cycle, 2$^2$ = 4 copies are produced. Similarly, after n$^{th}$ cycle, 2$^2$ copies are produced, where n is the number of cycles. Hence, after completion of 6 cycle, 2$^6$ = 64 copies will be produced.
Which of the following is not necessary to execute polymerase chain reaction successfully?
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All four DNA bases
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Short DNA base pairs
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DNA polymerase
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DNA library
Polymerase Chain Reaction (PCR) is widely used the method in genetic engineering to prepare the multiple copies of desired DNA. It includes steps of denaturation, annealing extension and last amplification. As DNA is the polymer of four DNA bases present in the base pair and polymerase chain reaction is done in the presence of DNA polymerase enzyme of the DNA that re-studies by the researchers which is said to be DNA library so it is not required in the reaction.
In reference to DNA polymerase III, which statement is wrong?
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It requires ATP For polymerase action.
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Required for PCR
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More active than DNA Pol I & II
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Requires a previously made template to work on
Polymerisation of DNA is in
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$3' \rightarrow 5'$ direction
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$5' \rightarrow 3'$ direction
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Both $3' \rightarrow 5'$ and $5' \rightarrow 3'$ direction
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None of the above