Tag: gene cloning
Questions Related to gene cloning
Population of cells derived from a single parent cell is called
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Symmetrical cell
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Diploid
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Haploid
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Clone
A large number of bacteria produced from a single bacterium is called as
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Deme
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Population
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Clones
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Offspring
'Cloning' is meant
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For production of HGH gene is E. coli
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To preserve the genotype of organism
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To replace the original gene
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All of the above
Two offsprings having the same genetic material and the same body features, produced by one parent through asexual reproduction, are called as
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Identical twins
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Fraternal twins
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Callus
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Clones
Which one of the following is used as vector for cloning genes into higher organisms?
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Retrovirus
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Baculovirus
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$Salmonella$ $typhimurium$
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$Rhizopus$ $nigricans$
Retroviruses, though have single-stranded RNA genomes but provide perhaps the most promising vector system of all. During the process of reverse transcription, sequences from the termini of viral RNA are duplicated to generate long terminal repeats(LTRs). These long terminal repeats contain both the promoter and the polyadenylation signal for the transcription of viral mRNAs. The specificity of proviral DNA integration is also determined by the long terminal repeats. Although retroviruses can integrate at many sites within the cellular genome, integrative recombination always occurs at particular sites at the ends of the LTRs. The sequences appropriately inserted between the two LTRs will be integrated intact which contrasts sharply with the integration of papovavirus or adenovirus DNA, during which extensive rearrangements of the integrated viral sequences are commonplace. A further great advantage of retroviruses is that they are natural transducing viruses.
Which is not a key step in the process of DNA molecular cloning?
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Heat shock at $42^oC$ for 90 seconds.
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Make DNA competent to take up plasmid with use of calcium chloride
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Seal plasmid carrying targeted genes into host cells genome using ligase.
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Introduce genes for cloning into a plasmid vector using restriction enzymes.
Plants having similar genotypes produced by plant breeding are called as
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Clone
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Haploid
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Autopolypoid
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Genome
Cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually. The term clone is used in horticulture to refer to descendants of a single plant which were produced by vegetative reproduction. Many horticultural plant cultivars are clones, derived from a single individual, multiplied by some process other than sexual reproduction.
Dolly is a product of
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Genetic engineering
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Animal cloning
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Cell fusion
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Gene therapy
Dolly was a female domestic sheep, and the first mammal cloned from an adult somatic cell, using the process of nuclear transfer ie, cloning me.method called Somatic Cell Nuclear Transfer or SCNT. A clone has the same DNA sequence as its parent and so they are genetically identical. In SCNT, the nucleus of an egg cell is removed and replaced with the nucleus of a donor adult cell. Since 99.9% of the cell’s DNA is contained in the nucleus, the resulting animal will share almost exactly the same DNA as the original donor cell. So, the correct answer is option B.
Somatic gene therapy causes ___________.
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Changes in sperm
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Changes in progeny
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Changes in body cell
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Changes in ovum
SCID is caused by defective gene coding for enzyme ______________.
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Adenosine deaminase
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Adenosine transaminase
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Adenosine transferase
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Guanosine transaminaseGuanosine deaminase
Adenosine deaminase deficiency SCID, commonly called ADA SCID, is a very rare genetic disorder. It is caused by a mutation in the gene that encodes a protein called adenosine deaminase (ADA).