Tag: gene cloning

Questions Related to gene cloning

Which is considered a superior method for identification of urine sugars?

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. Endoscopy

  2. Thin layer chromatography

  3. Electrocardiogram

  4. Tomography

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Correct Option: B
Explanation:
  • Thin-layer chromatography is a method that is used for the separation and identification of the amino acids and sugars. 
  • There are different substances that may be present in the urine which is excreted by an organism. 
  • Generally, the amount of sugar that is excreted by humans is less. 
  • In the case of diabetic patients, the sugar in the urine can be detected by this method of chromatography in which there is a thin layer of silica gel and the solvent is used with respect to the sugars. 
  • The solvent flows and allows the separation of the different molecules.
  • Thus, the correct answer is option B. 

Terminator seeds produced by giant seed companies using techniques of genetic engineering are in fact _________________.

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. Hybrid seeds which germinate in the frame's field but do not produce seeds in the next generation

  2. Hybrid seeds which give a very high yield

  3. Seed which germinate and produce incompatible gametes

  4. Seeds that develop into fertile plants which produce flog-terminable seeds

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Correct Option: A

Which one is being produced by genetically altered genes _________________.

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. Calcitonin

  2. Platelet derived growth factor

  3. Erythropoietin

  4. All the above

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Correct Option: D

Protein $\alpha$-1 antitrypsin is used to treat

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. Cancer

  2. Rheumatoid arthritis

  3. Alzheimers disease

  4. Emphysema

  5. ADA deficiency disease in children

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Correct Option: D

Transgenic hirudin is obtained from

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. Ocimum sanctum

  2. Brassica napus

  3. Potato

  4. Tomato

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Correct Option: B
Explanation:

Transgenic hirudin is obtained from Brassica napus.

Ti plasmid used in genetic engineering is obtained from _________________.

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. Bacillus thuzingiensis

  2. Agrobacterium rhizogenes

  3. Agrobacterium tumefaciens

  4. Pseudomonas syringae

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Correct Option: A

Which of the following statements is/are correct? 

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. The current interest in the manipulation of microbes, plants and animals has raised serious ethical issues.

  2. One possible risk of genetic engineering is the accidental production of antibiotic resistant microorganisms.

  3. Although risks are possible, genetic engineering offers more of a contribution to human welfare than threats.

  4. All of these.

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Correct Option: D
Explanation:

  • Several animal studies indicate serious health risks associated with genetically modified (GM) food including infertility, immune problems, accelerated aging, faulty insulin regulation, and changes in major organs and the gastrointestinal system.
  • One of the first cases of human benefit from genetic engineering was the production of human insulin in bacterial cells. Previous to this, diabetic patients were treated with pig insulin. Use of bacterially-derived human insulin has substantially reduced the number of animals used to produce insulin and also significantly reduces adverse reactions in diabetic patients as the molecule is identical to human insulin. Since the successful production of genetically engineered insulin, many other human proteins have been produced in this manner. This technology has improved millions of lives.

So, the correct option is 'All of these.'.

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/ vectors.
(i) Bacterium (ii) Plasmid (iii) Plasmodium (iv) Bacteriophage

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. (i), (ii) and (iv)

  2. (i) only

  3. (i) and (iii)

  4. (ii) and (iv)

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Correct Option: A

Manipulation of DNA to change its structure is called genetic engineering.

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. True

  2. False

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Correct Option: A
Explanation:

Genetic engineering is a technique to alter the chemistry of genetic material (DNA and RNA), to introduce these into the host organisms and thus change the phenotype and genetic makeup of the organism.

So, the statement is 'True'.

Research projects: 

botany breeding and biotechnology branches of biotechnology gene cloning biotechnology and genetic engineering

  1. cloning

  2. identical/ non identical twins

  3. triplets/ quadruplets

  4. surrogacy

  5. IVF

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Correct Option: A
Explanation:

Cloning is the process of generating a genetically identical copy of a cell or an organism. Cloning happens all the time in nature for example, when a cell replicates itself asexually without any genetic alteration or recombination.

So, the correct option is 'Cloning'.