Tag: bio-chemistry

Questions Related to bio-chemistry

Which technique is used in separating proteins from other fractions?

  1. Chromatography

  2. Autoradiography

  3. Electrophoresis

  4. Ultracentrifugation


Correct Option: D

Which of the following compounds used in density gradient centrifugation?

  1. Cholesterol

  2. Sucrose

  3. Glycine

  4. DNA


Correct Option: B

Which of the following is generally used for creating density gradient during centrifugation?

  1. NaCl

  2. KCl

  3. CsCl

  4. $MgCl _2$


Correct Option: C

Cells obtained from an organism were homogenised and centrifuged. A test indicated that the cells contained glycogen. If you are asked to find out as quickly as possible whether the cells were from a plant or an animal, you would

  1. Examine the centrifuge for presence of extracts of centrioles

  2. Answer immediately that the cells were from an animal source

  3. Examine the centrifuge for the presence of extracts of chloroplasts

  4. Answer immediately that the cells were from a plant source.


Correct Option: B

In order to sediment free ribosomes, centrifugation is required to be done at

  1. 300,000g

  2. 50,000g

  3. 12,000g

  4. 800g


Correct Option: A

Pure fractions of cellular components are isolated by

  1. Chromatography.

  2. Differential centrifugation.

  3. Scanning electron microscopy.

  4. X-ray diffraction.


Correct Option: B

Buoyant density centrifugation is carried out at

  1. 50,000g for 1-3 hours

  2. 50,000g for 20 hours

  3. 1,00,000g for 1-3 hours

  4. 1,00,000g for 20 hours


Correct Option: D

The common method to determine weight of proteins is

  1. X-ray diffraction.

  2. Ultracentrifugation.

  3. Classical chemistry.

  4. Both A and C.


Correct Option: B

At what speed, the mitochondria can be separated out by differential centrifugation?

  1. $200\times $g

  2. $500\times $ g

  3. $800\times$g

  4. $8000\times$g


Correct Option: D

Which is the technique separates proteins according to their molecular weight?

  1. PAGE

  2. Affinity chromatography

  3. Ion exchange chromatography

  4. Gel filtration


Correct Option: D