Tag: zoology

Quizzes (289) Questions (5869)

Questions Related to zoology

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Gene amplification requires besides sample DNA

  1. Nucleotides

  2. Nucleotides and polymerases

  3. Alternate heating and cooling

  4. Both B and C
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Polymerase chain reaction (PCR) is an amplification technique for cloning the specific or targeted parts of a DNA sequence to generate thousands to millions of copies of DNA of interest. This molecular “xeroxing” process involves heating and cooling samples in a precise thermal cycling pattern over ≈30 cycles. PCR uses a small amount of template DNA, two primers that flank the target sequence, nucleotides, and thermostable DNA polymerase to amplify a specific region of DNA.

So, the correct answer is 'Both B and C.'

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

In vitro repeated replication of selected DNA components is

  1. Gene amplification

  2. Gene replication

  3. Gene synthesis

  4. Pleiotropy
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Gene amplification can occur in situ, forming a so-called homogeneously staining region (HSR) in the normal chromosomal location of the gene (many copies of the gene are present as tandem repeats) or as so-called double minute chromosomes (extra chromosomal fragments of DNA containing multiple copies of the amplified gene). Gene amplification can also occur as an artifact when tumor cells are cultured in vitro.

So, the correct answer is 'Gene amplification.'

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Microsatellite sequences are repeat units with base pairs

  1. 1 - 6

  2. 5 - 30

  3. 11 - 60

  4. 20 - 120
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Microsatellites are polymorphic repeating units of 1–6 base pairs in length, found in human DNA. Microsatellites can be amplified for identification by PCR and can be used as molecular markers. Microsatellite analysis (MSA) is a PCR analysis of DNA in exfoliated urine cells.

So, the correct answer is '1-6.'

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Hypervariable repeats/minisatellite DNA sequences were discovered by

  1. Jeffreys et al

  2. Sharp and Roberts

  3. Parsons et al

  4. Sears and Sears
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Discovering their high level of variability, Sir Alec Jeffreys developed DNA fingerprinting based on minisatellites, solving the first immigration case by DNA in 1985, and the first forensic murder case, the Enderby murders in the United Kingdom, in 1986.

So, the correct answer is 'Jeffreys et al.'

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Satellite DNA contains

  1. Repetitive DNA

  2. Segments forming sn RNA

  3. Segments forming sc RNA

  4. Both B and C.
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Satellite DNA consists of arrays of tandemly repeating sequences that are widely present (>30%) in the genome. Satellite DNA is mainly present in heterochromatin or the tightly packed regions of chromosomes in centromeres, telomeres, and sometimes even in the euchromatin region (active region of the genome).

So, the correct answer is 'Repetitive DNA.'

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Which is different in related species?

  1. Position of centromeres

  2. Position of NOR

  3. Satellite DNA

  4. All the above
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Satellite DNA consists of arrays of tandemly repeating sequences that are widely present (>30%) in the genome. Satellite DNA is mainly present in heterochromatin or the tightly packed regions of chromosomes in centromeres, telomeres, and sometimes even in the euchromatin region (active region of the genome).

So, the correct answer is 'Satellite DNA.'