Tag: pcr
Questions Related to pcr
To carry out a polymerase chain reaction (PCR), one must have the catalytic DNA polymerase and
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a blueprint or gene map of the sequence one wish to copy
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a number of primers from either side of the target DNA in order to get the polymerase replication process- going
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a DNA synthesizer machine
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a DNA probe
Polymerase Chain Reaction is a method widely used to make many copies of a specific DNA segment. Using PCR, a single (or more) copy of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment. Components of PCR include DNA template, DNA polymerase, two DNA primers, nucleotides.
What is the function of the polymerase chain reaction in genetic engineering?
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Cut DNA into many fragments
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Carry DNA into a new cell
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Link together newly joined fragments of DNA
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Make millions of copies of a specific segment of DNA
Polymerase Chain Reaction is a method widely used to make many copies of a specific DNA segment. Using PCR, a single (or more) copy of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.
In the $PCR$ technology the $DNA$ segment is replicated over a billion times. This repeated replication is catalyzed by the enzyme.
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$DNA$ polymerase
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Taq polymerase
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$DNA$ dependent $RNA$ polymerase
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Primase
The taq polymerase enzyme is obtained from
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Thiobacillus ferroxidans
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Bacillus subtilis
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Pseudomonas putida
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Thermus aquaticus
Which one is a true statement regarding $DNA$ polymerase used in $PCR$
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it is used to ligate introduced $DNA$ in recipient cell
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it serves as a selectable marker
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it is isolated from a virus
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it remains active at high temperature
Which of the following steps is/are catalysed by Taq polymerase in a PCR?
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Denaturation of template DNA
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Annealing of primers to template DNA
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Extension of primer end on template DNA
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All of these
PCR is the short form for polymerase chain reaction which is the phenomenon which is used for amplification of the part-specific region of DNA as per our interest. Taq polymerase is the enzyme that attaches nucleotides together and help is the extension of primer end on the template DNA.
Which among the following is true for protein synthesis in bacteria?
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It involves all the three types of RNAs (m-RNA, t-RNA and r-RNA)
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It involves $3$ types of RNA polymerase
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It involves single type of RNA polymerase
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It involves RNA processing
Select the wrong pair
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RNA polymerase I - Sn RNA 5S rRNA, r-RNA
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RNA polymerase I - r-RNA
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RNA polymerase II - hnRNA
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RNA polymerase III - tRNA
Taq polymerase enzyme is used in
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Restriction mapping
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Gene cloning
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PCR
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All of the above
Taq polymerase is a heat stable enzyme used in the polymerase chain reaction (PCR) to amplify segments of DNA in the lab.
Which of the following would not be a valid reason to use PCR?
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Help in analyzing DNA of ancient remains of fossilized woolly mammoth
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Help in analyzing the genome of a virus
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Help in determining the DNA of a hair sample found at a crime scene
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Help in transferring DNA from a plasmid containing GFP into E.coli
Majorly the PCR is used in analyzing and determining purposes only not the transferring purposes. For transferring purpose vectors are used. In analyzing and determining purposes, the sample is limited i.e. if the sample gets destroyed no analysis can be carried out which can be a big problem in the investigation cases. So to have extra copies of the sample it is first amplified by PCR.