Tag: pcr

Questions Related to pcr

Taq polymerase is isolated from which bacteria?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. E. coli

  2. Bacillus thuringiensis

  3. Thermus aquaticus

  4. Agrobacterium

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Correct Option: C

Polymerase chain reaction (PCR) is used for

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. In vivo replication of specific DNA sequence using thermostable DNA polymerase

  2. In vitro synthesis of mRNAIn vivo synthesis of mRNA

  3. In vitro replication of specific DNA sequence using thermostable DNA polymerase

  4. Separation of DNA fragments according to their size

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Correct Option: C

Which one of the following is NOT a PCR-based molecular marker?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. RAPD

  2. AP-PCR

  3. DAF

  4. RELP

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Correct Option: A

DNA is amplified by __________________.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. PCR technique

  2. Southern Blotting technique

  3. Northern Blotting technique

  4. Nucleic acid hybridization technique

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Correct Option: A

Which one of the following enzymes is used in PCR reaction?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Taq polymerase

  2. RNA polymerase

  3. Rib nuclease

  4. End nuclease

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Correct Option: A

Polymerase chain reaction technique is used for.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. DNA amplification

  2. Screening gene libraries

  3. Synthesis of polymerase

  4. DNA sequencing

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Correct Option: A

The $C _2$ glycolate cycle is also known as.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. PCR

  2. PCO

  3. PPP

  4. EMP

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Correct Option: A

If the initial number of template DNA molecules in a PCR reaction is $1000$, the number of product DNA molecules at the end of $20$ cycles will be closest to.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. $10^3$

  2. $10^6$

  3. $10^9$

  4. $10^{12}$

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Correct Option: C

How many cycles of PCR are required if desired number of DNA molecules is 64 and starting material is single stranded DNA instead of ds DNA ?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. 5

  2. 64

  3. 7

  4. 30

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Correct Option: A

Which does not describe a function of the DNA polymerase molecule?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Recognise the free nucleotide that pairs with the base on the template strand of DNA

  2. Read the strand of template DNA and recognise the base there

  3. Proofread to ensure that the proper base has been incorporated

  4. Make the proper nucleotide to match with the base read on the template strand

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Correct Option: D
Explanation:

DNA polymerase is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building blocks of DNA. The function of DNA polymerase is to replicate, proofread and repair DNA.DNA polymerases add nucleotide bases only when an RNA primer, a short piece of RNA, is already present. 

So, the correct option is 'Make the proper nucleotide to match with the base read on the template strand'.