Tag: pcr

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Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Taq polymerase is isolated from which bacteria?

  1. E. coli

  2. Bacillus thuringiensis

  3. Thermus aquaticus

  4. Agrobacterium
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Taq polymerase is named after the bacterium Thermus aquaticus, which lives in hot springs and provides a heat-stable enzyme necessary for PCR.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction (PCR) is used for

  1. In vivo replication of specific DNA sequence using thermostable DNA polymerase

  2. In vitro synthesis of mRNAIn vivo synthesis of mRNA

  3. In vitro replication of specific DNA sequence using thermostable DNA polymerase

  4. Separation of DNA fragments according to their size
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

PCR is an in vitro technique used to amplify specific DNA sequences. It utilizes a thermostable DNA polymerase to synthesize multiple copies of the target DNA.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

DNA is amplified by __________________.

  1. PCR technique

  2. Southern Blotting technique

  3. Northern Blotting technique

  4. Nucleic acid hybridization technique
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

PCR (Polymerase Chain Reaction) is the standard laboratory technique used to amplify specific segments of DNA.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which one of the following enzymes is used in PCR reaction?

  1. Taq polymerase

  2. RNA polymerase

  3. Rib nuclease

  4. End nuclease
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Taq polymerase is the thermostable enzyme essential for the extension phase of the PCR reaction, allowing it to withstand high temperatures.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction technique is used for.

  1. DNA amplification

  2. Screening gene libraries

  3. Synthesis of polymerase

  4. DNA sequencing
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The primary purpose of the Polymerase Chain Reaction (PCR) is to create millions of copies of a specific DNA sequence, known as DNA amplification.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

If the initial number of template DNA molecules in a PCR reaction is $1000$, the number of product DNA molecules at the end of $20$ cycles will be closest to.

  1. $10^3$
  2. $10^6$
  3. $10^9$
  4. $10^{12}$
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

The number of DNA molecules after n cycles is calculated as N = N0 * 2^n. Here, N0 = 1000 and n = 20. So, N = 1000 * 2^20. Since 2^10 is approximately 10^3, 2^20 is approximately 10^6. Thus, 1000 * 10^6 = 10^9.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which does not describe a function of the DNA polymerase molecule?

  1. Recognise the free nucleotide that pairs with the base on the template strand of DNA

  2. Read the strand of template DNA and recognise the base there

  3. Proofread to ensure that the proper base has been incorporated

  4. Make the proper nucleotide to match with the base read on the template strand
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

DNA polymerase is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building blocks of DNA. The function of DNA polymerase is to replicate, proofread and repair DNA.DNA polymerases add nucleotide bases only when an RNA primer, a short piece of RNA, is already present. 

So, the correct option is 'Make the proper nucleotide to match with the base read on the template strand'.