Tag: pcr

Questions Related to pcr

Polymerase chain reaction employs

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Primers and DNA ligase

  2. DNA ligase only

  3. DNA polymerase only

  4. Primers and DNA polymerase

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Correct Option: D

DNA polymerase or taq enzyme used in PCR is isolated from

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Thermus aquaticus

  2. E. coli

  3. Salmonella typhimurium

  4. None of these

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Correct Option: A
Explanation:

Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus.


Source of Taq polymerase used in PCR is a

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Thermophilic fungus

  2. Mesophilic fungus

  3. Thermophilic bacterium

  4. Halophilic bacterium

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Correct Option: C
Explanation:

Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus.

Gene amplification using primers can be done by 

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Microinjection

  2. ELISA

  3. Polymerase chain reaction

  4. Gene gun

  5. Electrophoresis

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Correct Option: C

PCR proceeds in three distinct steps governed by temperature. The order of the steps is 

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Denaturation, Synthesis, Annealing

  2. Annealing, Synthesis, Denaturation

  3. Synthesis, Annealing, Denaturation

  4. Denaturation, Annealing, Synthesis

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Correct Option: D

What is true about DNA polymerase used in PCR?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. It is used to ligate introduced DNA in recipient cells.

  2. It serves as selectable marker.

  3. It is isolated from a virus.

  4. It is active at high temperature.

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Correct Option: D

Melting of DNA at 70$^o$ C is due to breakdown of

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Phosphodiester bonds

  2. Hydrogen bonds

  3. Glycosidic bonds

  4. Disulphide bonds

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Correct Option: B

Amplification of gene of interest by using PCR may go upto

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. 0.1 million

  2. 1.0 million

  3. 1.0 billion

  4. 1.0 trillion

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Correct Option: C

At which temperature a DNA molecule is denatured by heat in PCR

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. 70$^o$ -  80$^o$C

  2. 90$^o$ - 95$^o$C

  3. 42$^o$C

  4. 50$^o$ - 65$^o$C

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Correct Option: B

Taq polymerase is isolated from a

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Virus

  2. Bacterium

  3. Fungus

  4. Protozoan

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Correct Option: B