Tag: applications of biotechnology

Quizzes (11) Questions (246)

Questions Related to applications of biotechnology

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

All of the followings are associated with replacement principle of animal biotechnology except

  1. Cell culture

  2. Computer models

  3. Epidemiological studies

  4. Improving experimental technique
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

The replacement principle involves using non-animal alternatives. Improving experimental techniques is part of the 'Refinement' principle, not 'Replacement'.

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

First transgenic mouse grew twice the normal size after drinking -------- containing water.

  1. Cu

  2. Fe

  3. Zn

  4. Ra
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

When the transgenic mice were a few weeks old, they were given drinking water containing zinc. Mice carrying the transgene grew to twice the size of their litter mates because the metallothionein enhancer sequence stimulated by zinc had increased growth hormone production.

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

The Transgenic animals are generally produced for all of the following needs EXCEPT.

  1. Testing of chemical safety

  2. Testing of vaccine safety

  3. Stimulation of pathogenicity

  4. Production of pharmacologically important proteins
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Transgenic animals are used for testing chemical safety, testing vaccine safety, and producing pharmaceutical proteins. 'Stimulation of pathogenicity' is not a standard purpose - transgenic animals may model disease but not 'stimulate pathogenicity,' which is poorly phrased and unclear.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Enzyme' Taq polymerase' used in PCR, has been isolated from bacterium _________________.

  1. Agrobacterium tumefaciens

  2. Thermus aquaticus

  3. Streptomyces albus

  4. Escherichia coli
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The final step of PCR is the extension, wherein Taq DNA polymerase is isolated from a thermophilic bacterium Thermus aquaticus.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

The polymerase chain reaction is a technique used for ____________.

  1. Amplification of DNA

  2. Amplification of enzymes

  3. Amplification of proteins

  4. All of these
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Polymerase chain reaction is a technique used for amplification of DNA fragments. Its amplification cycle involve three steps - denaturation, annealing and polymerisation which are repeated for 'n', cycles.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following statements are correct for the enzyme Taq polymerase?


(i) It remains active during the high temperature-induced denaturation of dsDNA.

(ii) It requires primers for carrying out the process of polymerisation.
(iii) It synthesizes the RNA region between the primers, using $dNTPs$ and $Mg^{2+}$ _______________.

  1. (i) and (ii)

  2. (ii) and (iii)

  3. (i), (ii) and (iii)

  4. None of these
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The final step of PCR is the extension, wherein Taq DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesizes the DNA region between the primers, using dNTPs (deoxynucleoside triphosphates) and $Mg^{2+}$. The primers are extended towards each other so that the DNA segment lying between the two primers is copied. The optimum temperature for this polymerisation step is $72^o$$C$. Taq polymerase remains active during high temperature-induced denaturation of double-stranded DNA.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Process used for amplification or multiplication of DNA in DNA fingerprinting is

  1. Polymerase chain reaction

  2. Southern blotting

  3. Northern blotting

  4. None of these
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Process used for amplification or multiplication of DNA in DNA fingerprinting is Polymerase chain reaction (PCR) .It is done in invitro system.

So, the correct option is 'Option A' . 

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Primers are

  1. Chemically synthesised oligonucleotides that are complementary to the regions of DNA

  2. Chemically synthesised oligonucleotides that are not complementary to the regions of DNA

  3. Chemically synthesised, autonomously replicating circular DNA molecules

  4. Specific sequences present on recombinant DNA
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation
Primers are small, chemically synthesised oligonucleotides that are complementary to the sequences, present at 3' end of the template DNA. They hybridise to the target DNA region, one to each strand of the double helix. These primers are oriented with their ends facing each other allowing synthesis of the DNA towards one another.
So, the correct answer is 'Chemically synthesised oligonucleotides that are complementary to the regions of DNA'.
Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Given table gives an account of differences between PCR and gene cloning. Which of the, following points shows the incorrect difference?

  Parameter PCR Gene cloning
1 Efficient More Less
2 Apparatus Requirement DNA Restriction enzyme, ligase, vector, bacterial cell
3 Manipulation In vitro In vitro and in vivo
4 Cost More Less
5 Automation Yes No
6 Error probability Less More
7 Time for a typical experiment 2-4 days 4 hours
8 Application More Less


  1. 1 and 3

  2. 4 and 6

  3. 4 and 7

  4. 4, 7 and 8
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

The cost of gene cloning is far more than PCR because gene cloning requires many intricate steps. PCR takes less than 4 hours while gene cloning can take days.