Tag: applications of biotechnology

Questions Related to applications of biotechnology

Bioreactors are

  1. Transgenic animals

  2. Fermentation factories

  3. Both (A) and (B)

  4. None of the above


Correct Option: B
Explanation:

A bioreactor may refer to any manufactured or engineered device or system that supports a biologically active environment. In animals, the production of biologically active recombinant proteins use transgenic animals as bioreactor. Bioreactor or fermentation processes are the core manufacturing process in the biotech industry. 

In transgenics, expression of transgene in target tissue is determined by __________.

  1. Enhancer

  2. Transgene

  3. Promoter

  4. Reporter


Correct Option: D

Transgenic animal has

  1. Foreign DNA is all its cells

  2. Foreign RNA is all its cells

  3. Foreign DNA is some of the cells

  4. Both B and C


Correct Option: A
Explanation:

A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome. The foreign gene is constructed using recombinant DNA methodology. A transgenic organism carries in all its cells a foreign gene that was inserted by laboratory techniques. Each transgenic organism is produced by introducing cloned genes, composed of deoxyribonucleic acid (DNA) from microbes, animals or plants, into plant and animal cells. 

Thus, the correct answer is option A.

Reporter genes which are used in transformation are of types

  1. Selectable marker gene

  2. Scoreable marker gene

  3. Antibiotic resistant gene

  4. Both A and B


Correct Option: D

Which of the following is a function of a selectable marker?

  1. Provides resistance against a substrate

  2. Inhibits the growth of normal cell in a culture

  3. Help to create a chromosome map

  4. Both A and B


Correct Option: D

Which of the following ethical issues should be considered for developing transgenic animal?

  1. Yielding capacity

  2. Long term effect on environment

  3. Quality of breed

  4. All of the above


Correct Option: D
Explanation:

The ethical issues considered for developing transgenic animals are yielding capacity, long term effect on the environment, quality of the breed. They include bioethicists, environmentalists. the results of genetic alteration would be suffered and hence such experimentation should be banned (Eg, a pig with no legs)

Ethics are associated with genetic engineering because of ___________.

  1. Human dignity

  2. Commercialization of genetic research

  3. Fair use of genetic information

  4. All of the above


Correct Option: D
Explanation:

The genetic engineering of animals has increased significantly in recent years, and the use of this technology brings with it ethical issues, some of which relate to animal welfare — defined by the World Organisation for Animal Health as “the state of the animal.

How an animal is coping with the conditions in which it lives”.
These issues need to be considered by all stakeholders, including veterinarians, to ensure that all parties are aware of the ethical issues at stake and can make a valid contribution to the current debate regarding the creation and use of genetically engineered animals. 

Which of the following GM microbe contains gene for naphthalene?

  1. Mycobacterium tuberculosis

  2. E.coli

  3. Pseudomonas putida

  4. Pseudomonas aeruginosa


Correct Option: C

The enzymatic degradation of lactose in dairy product can reduce the__________.

  1. Casein

  2. Whey

  3. Microbes

  4. Acrylamide


Correct Option: D
Explanation:

Acrylamide does not form, or forms at lower levels, in dairy, meat, and fish products. so the enzymatic degradation of lactose in dairy produts can reduce the acrylamide.

What was the amount of Alpha-lactalbumin in the milk of transgenic cow?

  1. $2.4$ grams/lit

  2. $4.2$ grams/lit

  3. $3.4$ grams/lit

  4. $4.3$ grams/lit


Correct Option: A
Explanation:

Alpha-lactalbumin is a major milk whey protein. It is involved in the process of lactose synthesis. The first transgenic cow was produced with human alpha-lactalbumin gene introduced into it. The milk of transgenic cow contained about 2.4 grams/lit of human protein.

Thus, the correct answer is '2.4 grams/lit.'