Tag: application of biology in therapy and vaccine

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Multiple choice zoology applications of biotechnology genetically engineered insulin application of biology in therapy and vaccine application of biotechnology in medicine

Which of the following statements is not correct? 

  1. Insulin used for diabetic patients was earlier extracted from pancreas of slaughtered cattle and pigs which was more efficient than that the genetically engineered insulin

  2. PCR technique is applied to detect HIV in Suspected AIDS patients and to detect mutations in genes in suspected cancer patients

  3. Bone marrow transplantation requires periodic infusion of genetically engineered lymphocytes

  4. Bio-remediation is the one of the applications of biotechnology
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Earlier, insulin used for curing diabetes used to be extracted from pancreas of slaughtered pigs and cattle. This insulin was slightly different from human insulin and caused some undesirable side effects such as allergy. Genetically engineered insulin is a far more efficient.

Multiple choice zoology applications of biotechnology genetically engineered insulin application of biology in therapy and vaccine application of biotechnology in medicine

Select the correct options to fill up the blanks.
(i) ___enzymes is crucial for the immune system to function and its absence is caused by the deletion of a gene.
(ii) Insulin consists of _and _that are linked together by _.
(iii) Transgenic mice are being used to test the safety of the __.
(iv)___involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA. 

  1. (i) Adenosine deaminase (ii) A-chain, B-chain, disulfide bridges (iii) polio vaccine (iv) RNAi

  2. (i) RNAi (ii) A-chain, B-chain, disulphide bridges (iii) adenosine deaminase (iv) polio vaccine

  3. (i) Adenosine deaminase (ii) A-chain, B-chain, hydrogen bonds (iii) polio vaccine (iv) RNAi

  4. (i) RNAi (ii) A-chain, B-chain, non-covalent bridges (iii)polio vaccine (iv) adenosine deaminase
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

  • Adenosine deaminase enzymes is crucial for the immune system to function and its absence is caused by the deletion of a gene. It is cured by the gene therapy.
  • Insulin consists of chain A and chain B that are linked together by disulphide bridges. 
  •  Transgenic mice are being used to test the safety of the polio vaccine.
  • RNAi involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA. 
So, the correct option is 'Option A' .

Multiple choice zoology applications of biotechnology genetically engineered insulin application of biology in therapy and vaccine application of biotechnology in medicine

The first step in production of insulin using E.coli is

  1. Isolation of mRNA transcribing for insulin pancreas cell.

  2. Isolation of nucleotides transcribing for insulin from pancreas cell.

  3. Isolation of gene producing insulin from human DNA.

  4. Attachment of gene producing insulin from human DNA to plasmid using ligase.
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Scientists synthesized in the laboratory genes for the two insulin "A" and "B" chains. This is accomplished by chemically linking together small pieces of DNA sequence and then joining them in a specific manner to form complete genes. Once the genes were synthesized, they are attached into circular DNA strands called as plasmids. The newly constructed plasmids containing the transplanted genetic material were introduced into a benign E. coli bacterial strain.