Tag: option b: biotechnology and bioinformatics

Questions Related to option b: biotechnology and bioinformatics

Elution means

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Separation of DNA fragments on agarose gel

  2. Cutting and extraction of DNA bands from the agarose gel

  3. Making the DNA bands visible under UV radiation

  4. Isolation of alien DNA from the choice organism

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Correct Option: B

A dicotyledonous plant forms crown gall when

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Agrobacterium tumefaciens comes in contact with the plant

  2. Agrobacterium rhizogenes comes in contact with the plant

  3. A specific part of DNA from the Ti plasmid gets integrated with the plant chromosome

  4. A specific part of DNA from the Ri plasmid gets integrated with the plant chromosome

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Correct Option: C

Find the correct statement with respect to function or properties of RNA polymerase.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Bacteria have $3$ different types of RNA polymerase

  2. RNA polymerase binds to promoter and initiate transcription

  3. It uses nucleoside monophosphate as substrate

  4. RNA polymerase polymerizes in a template independent manner

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Correct Option: A

Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. $1, 3, 2, 4, 5$

  2. $3, 2, 1, 5, 4$

  3. $1, 3, 5, 4, 2$

  4. $5, 1, 3, 4, 2$

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Correct Option: A

When E coli with DNA $^{15}N ^{15}N $ is allowed to grow in a medium containing $^{14}N$ the number of DNA strands with $^{15}N$ after five generations is: 

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. 1

  2. 2

  3. 8

  4. 16

  5. 30

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Correct Option: A

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. Reverse transcriptase-production of.cDNA from mRNA

  2. DNA polymerase-used in polymerase chain reaction to amplify section of DNA

  3. DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments

  4. Restriction enzyme-production of RFLPs

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Correct Option: C
Explanation:

Restriction enzymes cut DNA creating sticky ends of restriction fragments. While DNA ligase joins these sticky ends to form recombinant DNA. 

So, the correct option is 'DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments'. 

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

botany option b: biotechnology and bioinformatics insertion and ligation of dna cutting, insertion and ligation of dna biotechnology in agriculture

  1. iii, iv

  2. i, iv

  3. i, ii

  4. ii, iii

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Correct Option: D

In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Silver or Tungsten

  2. Arsenic or Silver

  3. Gold or Tungsten

  4. None of these

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Correct Option: C
Explanation:
Biolistic method or gene gun method is a direct or vectorless method of introducing DNA into cells that involves bombardment of cells with high-velocity microprojectiles coated with DNA. In biolistic method, tungsten or gold particles, coated with foreign DNA are bombarded into target at a very high velocity.
So, the correct answer is 'Gold or tungsten'.

Which of the following techniques can be used to introduce foreign DNA into the cell?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Using disarmed pathogen

  2. Microinjection

  3. Gene gun

  4. All of these

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Correct Option: D
Explanation:

Vectors, gene gun ( biolistic ) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is All of these.

Electroporation is

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Making transient pores in cell membranes to introduce gene constructs

  2. Fast passage of nutrients through phloem sieve pores by electric stimulation

  3. Opening of stomata by artificial light during night

  4. Purification of saline water with the help of membrane system

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Correct Option: A