Tag: option b: biotechnology and bioinformatics

Questions Related to option b: biotechnology and bioinformatics

Genes can be inserted into human cells by

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. PCR

  2. Xenotransplantation

  3. Modified viruses

  4. Modified microarrays

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Correct Option: C

Find the odd one among the following methods of rDNA transfer into host cell.

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Biolistics

  2. Gene gun

  3. Microinjection

  4. Retroviral vector

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Correct Option: A
Explanation:

There are various methods of making the host cell competent for transformation with Recombinant DNA technology.

Microinjection method is one where the recommended DNA is directly injected into the nucleus of the animal cell. In the gene gun method, the bombardments on plant cell with high-velocity microparticles of gold or tungsten coated with DNA is done which is known as biolistic or gene gun.
The pathogen vectors are allowed to infect the cell and transfer the recombinant DNA into the host cell which is known as the retroviral vector method.
So, the correct option is 'Biolistics'.

Gene transfer is present in:-

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Biolistics

  2. Hybridization

  3. Tissue culture

  4. Vegetative propagation

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Correct Option: A

Which of the following is not a method of gene transfer in Bacteria?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Transformation

  2. Transduction

  3. Conjugation

  4. Gametangia contact

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Correct Option: A

Which cannot be used for direct gene transfer 

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Biolistics (gene gun)

  2. Microinjection

  3. Electroporation

  4. Agrobacterium tumefacians

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Correct Option: D
Explanation:
  • Biolistics (gene gun), Microinjection and Electroporation are direct methods of gene transfer.
  • Agrobacterium tumefaciens is a gram-negative soil bacterium, which can cause crown gall tumors at wound sites of infected dicotyledonous plants.
  • Agrobacterium tumefaciens acts as a vector for the transfer of genetic material hence it cannot be used for direct gene transfer.
  • Hence Agrobacterium tumefaciens cannot be used for direct gene transfer.
  • So, the correct answer is 'Agrobacterium tumefaciens'.

Which of these is used as vector in gene therapy for SCID?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Arbovirus

  2. Rotavirus

  3. Enterovirus

  4. Parvovirus

  5. Retrovirus

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Correct Option: E
Explanation:

In gene therapy trials, the retrovirus is used as a vector to treat X-linked severe combined immunodeficiency (X-SCID) represent the most successful application of gene therapy. The retrovirus genetic material is in the form of RNA molecules, and their hosts genetic material is in the form of DNA. When a retrovirus infects a host cell, it will introduce its RNA together with some enzymes, namely reverse transcriptase and integrase, into the cell. This RNA molecule from the retrovirus must produce a DNA copy from its RNA molecule before it can be integrated into the genetic material of the host cell. The process of producing a DNA copy from an RNA molecule is termed reverse transcription.

Transfer of DNA bands from agarose gel to nitrocellulose or nylon membrane is

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Southern transfer

  2. Western transfer

  3. Northern transfer

  4. Eastern transfer

  5. Gene transfer

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Correct Option: A

Gene gun can introduce genes into cells with the help of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Plasmids

  2. Cosmids

  3. Microscopic pellets

  4. Phagemids

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Correct Option: C
Explanation:

In the gene gun method, microscopic pellets of gold or tungsten are coated with the transgene fragments and shot into the plant cells or tissues at high velocity. 

So the correct option is C.

The technique in which foreign DNA is precipitated over surface of metal particles for passing into target cells is __________.

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Microinjection

  2. Electroporation

  3. Particle gun

  4. Chemical mediated gene transfer

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Correct Option: C

Microparticles for coating with DNA to be bombarded with gene gun are made of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Silver or platinum

  2. Platinum or zinc

  3. Silicon or platinum

  4. Gold or tungsten

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Correct Option: D