Tag: option b: biotechnology and bioinformatics

Quizzes (20) Questions (191)

Questions Related to option b: biotechnology and bioinformatics

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Plasmids are suitable vectors for gene cloning because they are

  1. Small circular DNA molecules with their own origin of replication site

  2. Small circular DNA molecules which can integrate with host chromosomal DNA

  3. Having antibiotic genes

  4. Able to shuttle between prokaryotic and eukaryotic cells
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Plasmids are ideal vectors because they are small, circular, and contain an origin of replication, which ensures they can be maintained and copied by the host cell.

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Plasmids are used as vectors in genetic engineering because of their

  1. Resistance to antibiotics

  2. Resistance to restriction enzymes

  3. Ability to carry foreign genes

  4. Ability to cause infection in host
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Plasmids are used as vectors because they can be engineered to carry foreign genes and replicate within a host organism.

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Insect tolerant gene from Bacillus thuringiensis is introduced using $T 1$ plasmid of _______.

  1. Escherichia coli

  2. Agrobacterium tumefaciens

  3. Haemophilus influenzae

  4. Arabidopsis thaliana
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The Ti plasmid of Agrobacterium tumefaciens is the standard tool used to introduce insect-resistant genes, such as those from Bacillus thuringiensis, into plants.

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Which of the following is correctly matched?

  1. Agrobacterium tumefaciens- Tumour

  2. pBR 322- Enzyme

  3. Thermus aquaticus- Bt gene

  4. Ligase- Molecular scissors

  5. Hind II- Plasmid vector
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Agrobacterium tumefaciens contains a Ti plasmid that causes crown gall disease (tumors) in plants. Other options are incorrectly matched; for example, Hind II is a restriction enzyme, not a vector.

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Pathogenic entity that is responsible for the production of tumour in most dicot plants is

  1. Retrovirus

  2. Bacteriophage

  3. Ti plasmid

  4. Vector
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Agrobacterium is a genus of Gram-negative bacteria that causes tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus. It contains "Ti" plasmid or tumor inducing plasmid, that causes  crown-gall disease in plants. The disease is characterised by a tumour-like growth or gall on the infected plant, often at the junction between the root and the shoot. Tumors are incited by the conjugative transfer of a DNA segment (T-DNA) from the bacterial tumour-inducing (Ti) plasmid to the plant cell naturally when the bacterium infects the plant through  a wound.


So, the correct answer is 'Ti plasmid'

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

Diseases like malaria and filariasis are transmitted through insect vectors .Vectors can be controlled by introducing fish

  1. Labeo

  2. Gambusia

  3. Hilsa

  4. Catla
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Gambusia is a larvivorous fish used as a biological control agent to eat mosquito larvae, thereby reducing the transmission of diseases like malaria.

Multiple choice botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

pBR $322$ which is frequently used as a vector for cloning gene in E.coli is?

  1. An original bacterial plasmid

  2. A modified bacterial plasmid

  3. A viral genome

  4. A transposon
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Plasmids like pBR322 are not naturally occurring raw plasmids found directly in bacteria in that exact form. Instead, they are artificially constructed cloning vectors derived by modifying natural bacterial plasmids to include antibiotic resistance genes and unique restriction sites.