Tag: biochemical techniques

Questions Related to biochemical techniques

Agarose gel used to separate

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Carbohydrates.

  2. Fats.

  3. Proteins.

  4. Both A and B.

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Correct Option: C

Electric field is used in separating chemicals in which method?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Ion exchange chromatography

  2. Electrophoresis

  3. Thin layer chromatography

  4. Column chromatography

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Correct Option: B

Electrophoresis is employed for

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Separating cell components.

  2. Separating charged particles.

  3. Reverse osmosis.

  4. In vitro storage of cell components.

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Correct Option: A

The base material that can be used in electrophoresis is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Polyacrylamide.

  2. Agarose.

  3. Carrageenan.

  4. Both A and B.

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Correct Option: D

In DNA segment of six coils, 22 bp are linked by two hydrogen bonds. How many cytosine bases would be present

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. 22

  2. 38

  3. 44

  4. 76

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Correct Option: B

In a DNA fragment, there are 8 turns with 40% of the bases are cytosine. What would be the number of hydrogen bonds present in this DNA fragment 

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. 96

  2. 192

  3. 224

  4. 60

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Correct Option: C

Which one of the following statements is wrong with respect to separation of DNA fragments on gel electrophoresis

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. DNA fragments move towards anode under electric field through the matrix

  2. The commonly used matrix is agarose gel

  3. DNA fragments resolve according to their size

  4. The smaller DNA fragments separate first

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Correct Option: D

Escherichia coli, in which both the strands of DNA are labeled  with $^{15}N$ is transferred to $^{14}N$ medium and allowed to replicate for three generations. Find out the number of hybrid DNA molecules in the third generation

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. 8

  2. 2

  3. 12

  4. 10

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Correct Option: B

Fragments of DNA formed after treatment with endonucleases are separated by the technique

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Polymerase chain reaction

  2. Southern blotting

  3. Colony hybridisation

  4. Electrophoresis

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Correct Option: D

First step in Southern blot technique is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Digestion of DNA by restriction enzyme

  2. Production of a group of genetically identical cells

  3. Denaturation of DNA on the gel for hybridisation with specific probe

  4. Denaturation of DNA from a nucleated cell as from the scene of crime

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Correct Option: A
Explanation:

The first step in a Southern blot technique is to prepare the DNA mixture by breaking it into smaller fragments using a restriction enzyme.