Tag: biochemical techniques

Questions Related to biochemical techniques

Centrifugation is used for :

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. separation of miscible liquids

  2. separation of immiscible liquids

  3. separation of immiscible gases

  4. all of above

Show answer
Correct Option: B
Explanation:

Centrifugation is used to separate two immiscible substances. More dense components of the mixture migrate away from the axis of the centrifuge, while less dense components of the mixture migrate towards the axis. 

Centrifugation is a process which involves the application of the :

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. centripetal force

  2. gravitational force

  3. friction

  4. none of above

Show answer
Correct Option: A
Explanation:

Centrifugation is a process which involves the application of the centripetal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings.

When milk is churned rapidly, _______ migrates away from the centre of centrifuge.

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. fat

  2. protein

  3. water

  4. sugar

Show answer
Correct Option: C
Explanation:

When milk is churned rapidly, water which is lighter than fat, migrates away from the centre of centrifuge while fat is forced towards the bottom, which is drained out.

Which of the following is an application of centrifugation?

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Pathological test of blood and urine

  2. Preparing lactic cultures

  3. Washing machine

  4. All of above

Show answer
Correct Option: D
Explanation:

In the method of centrifugation, the centripetal and centrifugal forces are used to separate lighter and heavier components of mixture of two immiscible liquids. It can be used in pathological test of blood and urine, in washing machines to squeeze the water from wet clothes and to prepare lactic cultures for production of cheese in dairies.

In a centrifuge, more-dense components of the mixture__of the centrifuge, while less-dense components of the mixture ___.       

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. migrate away from the axis, migrate towards the axis

  2. migrate towards the axis, migrate away from the axis

  3. both a and b

  4. none of above

Show answer
Correct Option: A
Explanation:

Centrifugation is a process which involves the application of the centripetal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings. This process is used to separate two immiscible substances. More-dense components of the mixture migrate away from the axis of the centrifuge, while less-dense components of the mixture migrate towards the axis. 

Centrifuge can be used to separate ___________.

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. oil and water

  2. mixture of solids

  3. two forms of uranium

  4. all of the above

Show answer
Correct Option: C
Explanation:

Centrifuge can be used to separate two forms of uranium. The process is the centrifugal separation of isotopes. The difference in the weights of two isotopes is used for the separation.

What forces out the water from fabric in washing machine?

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Magnetic force

  2. Frictional force

  3. Force of attraction

  4. Centrifugal force

Show answer
Correct Option: D
Explanation:

Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. When you wash clothes in a washing machine, it is the centrifugal force generated in the "spin" cycle that forces water out of the fabric to facilitate faster drying.

Colloids may be more than a thousand times as big as an atom but are more than a thousand times smaller than an ant. These colloids can be removed from a solution by:

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Thin piece of cloth

  2. Centrifuge

  3. Filter paper

  4. Parchment paper

Show answer
Correct Option: B
Explanation:

Particles of colloids range from $1-100$ $nm$ which is very small than pores of filter paper, thin piece of cloth or parchment paper so can't be separated by them but can be separated by ultra filtration and centrifuge

A mixture containing DNA fragments, A, B, C and D, with molecular weights of $A + B =C, A>B$ and $D>C$, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anoe sides of the gel would be

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. $D, C, A, B$

  2. $A, B, C, D$

  3. $C, B, A, D$

  4. $B, A, D, C$

Show answer
Correct Option: A
Explanation:

Gel electrophoresis is the techniques by which negatively charged DNA fragments separate according to their molecular mass and the principle says that lighter goes the maximum distance that means heavy DNA stops first as D is heavier it separates first while B at last.

So the correct option is 'D,C,A,B'.

Gel electrophoresis is a

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Technique of separation of charged molecules under the influence of magnetic field

  2. Technique of incorporation of DNA molecules into the cell through transient pores made due to electrical impulses

  3. Technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field

  4. Technique of separation and purification of gene products

Show answer
Correct Option: C
Explanation:
Electrophoresis is a technique of separation of molecules such as DNA, RNA or protein, under the influence of an electrical field, so that they migrate in the direction of electrode bearing the opposite charge, viz. positively charged molecules move towards cathode (-ve electrode) and negatively charged molecules travel towards anode (+ve electrode) through a medium/matrix. Since DNA fragments are negatively charged molecules, they can be separated by allowing them to move towards the anode (+ve electrode) under an electric field through a matrix of agarose gel.
So, the correct answer is 'Technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field'.