Tag: biochemical techniques

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Questions Related to biochemical techniques

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Centrifugation is used for :

  1. separation of miscible liquids

  2. separation of immiscible liquids

  3. separation of immiscible gases

  4. all of above
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Centrifugation is used to separate two immiscible substances. More dense components of the mixture migrate away from the axis of the centrifuge, while less dense components of the mixture migrate towards the axis. 

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Centrifugation is a process which involves the application of the :

  1. centripetal force

  2. gravitational force

  3. friction

  4. none of above
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Centrifugation is a process which involves the application of the centripetal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Which of the following is an application of centrifugation?

  1. Pathological test of blood and urine

  2. Preparing lactic cultures

  3. Washing machine

  4. All of above
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

In the method of centrifugation, the centripetal and centrifugal forces are used to separate lighter and heavier components of mixture of two immiscible liquids. It can be used in pathological test of blood and urine, in washing machines to squeeze the water from wet clothes and to prepare lactic cultures for production of cheese in dairies.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

In a centrifuge, more-dense components of the mixture__of the centrifuge, while less-dense components of the mixture ___.       

  1. migrate away from the axis, migrate towards the axis

  2. migrate towards the axis, migrate away from the axis

  3. both a and b

  4. none of above
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Centrifugation is a process which involves the application of the centripetal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings. This process is used to separate two immiscible substances. More-dense components of the mixture migrate away from the axis of the centrifuge, while less-dense components of the mixture migrate towards the axis. 

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

What forces out the water from fabric in washing machine?

  1. Magnetic force

  2. Frictional force

  3. Force of attraction

  4. Centrifugal force
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. When you wash clothes in a washing machine, it is the centrifugal force generated in the "spin" cycle that forces water out of the fabric to facilitate faster drying.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Colloids may be more than a thousand times as big as an atom but are more than a thousand times smaller than an ant. These colloids can be removed from a solution by:

  1. Thin piece of cloth

  2. Centrifuge

  3. Filter paper

  4. Parchment paper
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Particles of colloids range from $1-100$ $nm$ which is very small than pores of filter paper, thin piece of cloth or parchment paper so can't be separated by them but can be separated by ultra filtration and centrifuge

Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

A mixture containing DNA fragments, A, B, C and D, with molecular weights of $A + B =C, A>B$ and $D>C$, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anoe sides of the gel would be

  1. $D, C, A, B$
  2. $A, B, C, D$
  3. $C, B, A, D$
  4. $B, A, D, C$
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Gel electrophoresis is the techniques by which negatively charged DNA fragments separate according to their molecular mass and the principle says that lighter goes the maximum distance that means heavy DNA stops first as D is heavier it separates first while B at last.

So the correct option is 'D,C,A,B'.

Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

Gel electrophoresis is a

  1. Technique of separation of charged molecules under the influence of magnetic field

  2. Technique of incorporation of DNA molecules into the cell through transient pores made due to electrical impulses

  3. Technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field

  4. Technique of separation and purification of gene products
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation
Electrophoresis is a technique of separation of molecules such as DNA, RNA or protein, under the influence of an electrical field, so that they migrate in the direction of electrode bearing the opposite charge, viz. positively charged molecules move towards cathode (-ve electrode) and negatively charged molecules travel towards anode (+ve electrode) through a medium/matrix. Since DNA fragments are negatively charged molecules, they can be separated by allowing them to move towards the anode (+ve electrode) under an electric field through a matrix of agarose gel.
So, the correct answer is 'Technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field'.