Tag: biochemical techniques

Questions Related to biochemical techniques

Pure fractions of cellular components are isolated by

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. Chromatography.

  2. Differential centrifugation.

  3. Scanning electron microscopy.

  4. X-ray diffraction.

Show answer
Correct Option: B

Buoyant density centrifugation is carried out at

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. 50,000g for 1-3 hours

  2. 50,000g for 20 hours

  3. 1,00,000g for 1-3 hours

  4. 1,00,000g for 20 hours

Show answer
Correct Option: D

The common method to determine weight of proteins is

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. X-ray diffraction.

  2. Ultracentrifugation.

  3. Classical chemistry.

  4. Both A and C.

Show answer
Correct Option: B

At what speed, the mitochondria can be separated out by differential centrifugation?

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. $200\times $g

  2. $500\times $ g

  3. $800\times$g

  4. $8000\times$g

Show answer
Correct Option: D

Which is the technique separates proteins according to their molecular weight?

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. PAGE

  2. Affinity chromatography

  3. Ion exchange chromatography

  4. Gel filtration

Show answer
Correct Option: D

Heavier and lighter isotropic forms of an element can be separated by which technique?

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. Electrophoresis

  2. Density gradient centrifugation

  3. Buoyant density centrifugatio

  4. Chromatography

Show answer
Correct Option: C
Explanation:

Buoyant density centrifugation 

It is also called isopycnie centrifugation or equilibrium density - gradient centrifugation which uses the concept of Buoyancy to separate molecules in solutions by their difference in density.

Name the equipment used in measuring thermal behaviour of water _______________.

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. SEM

  2. Differential scanning calorimeter

  3. Real time PCR

  4. MALDI-TOF

Show answer
Correct Option: B

Pure fractions of cellular components can be isolated by

spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

  1. Chromatography

  2. Scanning electron microscopy

  3. X-ray diffraction

  4. Differential centrifugation

  5. Autoradiography

Show answer
Correct Option: D

Which one of the following reagent is useful for separating aniline from nitrobenzene?

methods of purification of organic compounds methods of purification - organic compounds organic chemistry - some basic principles and techniques organic chemistry : some basic principles and techniques chemistry biochemical techniques

  1. $ aq. NaHCO _3 $

  2. $ aq. NaHSO _3 $

  3. $ aq. HCl $

  4. $dil. H _2SO _4 $

Show answer
Correct Option: C
Explanation:

The reagent selected should be such that only one of components to be separated, reacts with it.


Aniline $+$ aq, HCl $\rightarrow$ salt, which is soluble in water
Nitrobenzene $+$ aq. HCl$\rightarrow$ no reaction.

Therefore, aq. HCL is used to separate aniline and nitrobenzene.

 A mixture of two immiscible liquids chlorobenzene and water boils at $90^{0}C$ and an external pressure of 740.2 torr. If vapour pressure of pure water at $90^{0}C$ is 530 torr then the mole % of chlorobenzene in the distillate will be:

methods of purification of organic compounds methods of purification - organic compounds organic chemistry - some basic principles and techniques organic chemistry : some basic principles and techniques chemistry biochemical techniques

  1. 80%

  2. 40%

  3. 28%

  4. 60%

Show answer
Correct Option: C