Tag: biochemical techniques

Quizzes (6) Questions (139)

Questions Related to biochemical techniques

Multiple choice spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

Pure fractions of cellular components are isolated by

  1. Chromatography.

  2. Differential centrifugation.

  3. Scanning electron microscopy.

  4. X-ray diffraction.
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Differential centrifugation is the standard method for separating cellular components based on their size and density by applying increasing centrifugal forces.

Multiple choice spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

Buoyant density centrifugation is carried out at

  1. 50,000g for 1-3 hours

  2. 50,000g for 20 hours

  3. 1,00,000g for 1-3 hours

  4. 1,00,000g for 20 hours
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Buoyant density centrifugation (equilibrium centrifugation) requires long run times, often 20 hours or more, at high speeds (like 100,000g) to allow the particles to reach their isopycnic position in the gradient.

Multiple choice spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

At what speed, the mitochondria can be separated out by differential centrifugation?

  1. $200\times $g
  2. $500\times $ g
  3. $800\times$g
  4. $8000\times$g
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Mitochondria are relatively large organelles that sediment at intermediate speeds, typically around 8,000g to 10,000g in a differential centrifugation protocol.

Multiple choice spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

Heavier and lighter isotropic forms of an element can be separated by which technique?

  1. Electrophoresis

  2. Density gradient centrifugation

  3. Buoyant density centrifugatio

  4. Chromatography
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Buoyant density centrifugation 

It is also called isopycnie centrifugation or equilibrium density - gradient centrifugation which uses the concept of Buoyancy to separate molecules in solutions by their difference in density.

Multiple choice spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

Name the equipment used in measuring thermal behaviour of water _______________.

  1. SEM

  2. Differential scanning calorimeter

  3. Real time PCR

  4. MALDI-TOF
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Differential scanning calorimetry (DSC) is the standard analytical technique used to measure the thermal behavior, such as heat capacity and phase transitions, of materials including water.

Multiple choice spectrophotometer, colorimeter and biosensor biochemical techniques bio-chemistry

Pure fractions of cellular components can be isolated by

  1. Chromatography

  2. Scanning electron microscopy

  3. X-ray diffraction

  4. Differential centrifugation

  5. Autoradiography
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Differential centrifugation is the primary laboratory technique used to isolate pure fractions of cellular components by exploiting differences in sedimentation rates.

Multiple choice methods of purification of organic compounds methods of purification - organic compounds organic chemistry - some basic principles and techniques organic chemistry : some basic principles and techniques chemistry biochemical techniques

Which one of the following reagent is useful for separating aniline from nitrobenzene?

  1. $ aq. NaHCO _3 $
  2. $ aq. NaHSO _3 $
  3. $ aq. HCl $
  4. $dil. H _2SO _4 $
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

The reagent selected should be such that only one of components to be separated, reacts with it.


Aniline $+$ aq, HCl $\rightarrow$ salt, which is soluble in water
Nitrobenzene $+$ aq. HCl$\rightarrow$ no reaction.

Therefore, aq. HCL is used to separate aniline and nitrobenzene.

Multiple choice methods of purification of organic compounds methods of purification - organic compounds organic chemistry - some basic principles and techniques organic chemistry : some basic principles and techniques chemistry biochemical techniques

 A mixture of two immiscible liquids chlorobenzene and water boils at $90^{0}C$ and an external pressure of 740.2 torr. If vapour pressure of pure water at $90^{0}C$ is 530 torr then the mole % of chlorobenzene in the distillate will be:

  1. 80%

  2. 40%

  3. 28%

  4. 60%
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Using Dalton's law for steam distillation: P_total = P_water + P_chlorobenzene. 740.2 = 530 + P_chlorobenzene, so P_chlorobenzene = 210.2 torr. The mole ratio n_c/n_w = P_c/P_w = 210.2/530 = 0.396. Mole fraction of chlorobenzene = 0.396 / (1 + 0.396) = 0.283, or 28%.