Tag: biochemical techniques

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Questions Related to biochemical techniques

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Nowadays we get full cream, toned and double-toned varieties of milk placed in polypacks or tetra packs in the market. What is the basic concept behind this?

  1. Distillation

  2. Centrifugation

  3. Decantation

  4. Sedimentation
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Centrifugation method is also used for separating cream from milk. When milk is rotated at high speed, the cream collects at the centre and is removed through an outlet.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Centrifugation is used for :
(a) particles which can't be seperated by filteration.
(b) particles which pass through a filter paper.
(c) seperating cream from milk.

  1. a, b, c

  2. a, b

  3. a, c

  4. b, c
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Centrifugation is a technique of separation cream from milk. This is used for particles which are very small and can easily pass through filter paper for particles which can't be seperated by filtration.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

In centrifugation :

  1. denser particles settle down

  2. lights particles stay at it top

  3. there are no distinction between lights or denser particles

  4. Both A and B
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

In centrifugation, denser particles settle down at the bottom of it container and the lighter particles stay at the top. Suspended particles are rotated at a high speed.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Principle of centrifugation is :

  1. denser particles stay at the top and the lighter particles are forced to the bottom

  2. lighter particle stay at the top and the denser particles are forced to the bottom

  3. high speed rotation increases the velocity of particles and make them charged

  4. particles become weightless due to high speed rotation
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The principle behind centrifugation is that the denser particles are forced to the bottom and the lighter particles stay at it top when spin rapidly.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

A ___________ is a centrifugal device that separates milk into cream and skimmed milk.

  1. condenser

  2. sublime

  3. separator

  4. all of the above
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

A separator is a centrifugal device that separates milk into cream and skimmed milk. When spun, the heavier milk is pulled outward against the walls of the separator and the cream, which is lighter, collects in the middle.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Blood cells are separated from plasma (the liquid part of blood) by :

  1. distillation

  2. centrifugation

  3. sedimentation

  4. chromatography
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

A centrifuge is a spinning machine, a bit like a clothes dryer, that separates materials. A mixture of liquids and solids is spun at high speed in a tube. The larger, denser particles sink and collect at the bottom. Light particles collect at the top. Blood cells are separated from plasma (the liquid part of blood) by this method.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Butter from curd can be removed by :

  1. sedimentation

  2. centrifugation

  3. filteration

  4. all of above
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

In the method of centrifugation, the centripetal and centrifugal forces are used to separate lighter and heavier components of mixture of two immiscible liquids. When curd is centrifuged, water which is lighter than fat, migrates away from the centre of centrifuge while fat is forced towards the bottom, which is drained out.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

Which of the following is a better method than filtration for separation of very small solids?

  1. Distillation

  2. Chromatography

  3. Separating funnel

  4. Centrifugation
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Sometimes the solid particles in a liquid are very small and can pass through a filter paper. For such particles, the filtration technique cannot be used for separation. Such mixtures are separated by centrifugation.

Multiple choice bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

The centrifuge consists of a centrifuge tube holder called :

  1. motor

  2. rotor

  3. moving tube

  4. both a and b
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The apparatus used for centrifugation is called a centrifuge. The centrifuge consists of a centrifuge tube holder called rotor. The rotor holds balanced centrifugal tubes of equal amounts of the solid-liquid mixture.