Tag: biochemical techniques

Questions Related to biochemical techniques

In analytical ultracentrifuges the maximum speed is:

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. $70000\ rpm$

  2. $7000\ rpm$

  3. $700000\ rpm$

  4. $1000\ rpm$

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Correct Option: A
Explanation:

In analytical ultracentrifuges the maximum speed is 70000 rpm.

Hence, option A is correct.

The subcellular organelles of a cell suspension can be isolated by __________________ technique.

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. centrifugation

  2. distillation

  3. chromatography

  4. none of the above

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Correct Option: A
Explanation:

Cenrifugation technique can be used to isolate subcellular organelles of a cell suspension.

Centrifugation is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge used in industry.

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. True

  2. False

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Correct Option: A
Explanation:

Centrifugation is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge used in industry and in laboratory settings. This process is used to separate two immiscible liquids.

Which of the following processes is used to separate cream from milk?

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Filtration

  2. Distillation

  3. Chromatography

  4. Centrifugal separator

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Correct Option: D
Explanation:

The separation of cream from milk in the centrifugal separator is based on the fact that when liquids of different specific gravities revolve around the same centre at the same distance with the same angular velocity, a greater centrifugal force is exerted on the heavier liquid than on the lighter one. Milk can be regarded as two liquids of different specific gravities, the serum and the fat.

What type of mixture are separated by centrifugation?

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Separating chalk powder from water

  2. Removing fat from milk to produce skimmed milk

  3. Separating water from textiles

  4. All of these

Show answer
Correct Option: D
Explanation:

Centrifugation is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge, used in industry and in laboratory settings. This process is used to separate two immiscible liquids.
Uses:

  • Separating chalk powder from the water.
  • Removing fat from milk to produce skimmed milk.
  • Separating water from textiles
  • Removing water from lettuce after washing it in a salad spinner.
  • Separating particles from an air-flow using cyclonic separation.
  • The clarification and stabilization of wine.
  • Separation of water particles from clothes while spin-drying in washing machines.
  • Separation of urine components and blood components in forensic and research laboratory.

Which of the following devices uses the principle of centrifugation?

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Washing machine

  2. Ceramic water filter

  3. Household ultraviolet (UV) purifier

  4. Distillation tower

Show answer
Correct Option: A
Explanation:

Centrifugation is a technique which involves the application of centrifugal force to separate particles from a solution according to their size, shape, density, the viscosity of the medium and rotor speed. The larger the size and the larger the density of the particles, the faster they separate from the mixture. Example- washing machine works on the principle of centrifugation.

A very large quantity of mixture contains tiny particles of three different substances X, Y, and Z. They are of the same size, cubical in shape and yellow in colour. X particles are very heavy, insoluble, non-magnetic and contribute 50% of the mixture. Y particles are very light, insoluble, non-magnetic and contribute 40% of the mixture. And Z particles are actually iron pieces. Which of the following methods can separate X, Y?
bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Distillation

  2. Decantation

  3. Centrifugation

  4. None of the above

Show answer
Correct Option: C
Explanation:

Particle X and Y are having same size, shape and colour but they are having different masses, 

particle X is heavy whereas particle Y is light,
so, based on this particle X and Y can be separated by centrifugation.

The method that is used to separate water particles from clothes while drying in washing machines is:

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. magnetic separation

  2. filtration

  3. evaporation

  4. centrifugation

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Correct Option: D

If the constituents of a solid-liquid mixture have different masses, then we can separate them using:

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. sublimation

  2. centrifugation

  3. winnowing

  4. evaporation

Show answer
Correct Option: B
Explanation:

Centrifugation is a separation process which uses the action of centrifugal force to promote accelerated settling of particles in a solid-liquid mixture. Two distinct major phases are formed in the vessel during centrifugation .It is best method for separating of mixture have different masses for example removing of cream from milk.

Which of the following devices uses the process of centrifugation?

bio-chemistry biochemical techniques centrifugation separation techniques separation of matter

  1. Washing machine

  2. Ceramic water filter

  3. Household ultraviolet (UV) purifier

  4. Distillation tower

Show answer
Correct Option: A
Explanation:

In the process of centrifugation, we use a rotator and because of that the heavier particles in the solution get settled at the bottom and we get a clear solution, in washing machine we are having rotator at the bottom and hence it can be used for the process of centrifugation.