The human genome project involves all of the following except
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altering the human genome
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physical mapping of the chromosomes
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sequencing of the entire nucleotide sequence of the human genome
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location of RFLP markers
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Explanation
The Human Genome Project (HGP) was launched on 1st October 1990 for sequencing the entire genome of 2.75 billion nucleotide pairs. This megaproject was a 13-year project coordinated by the U.S. Department of Energy and the National Institute of Health. During the early years of the HGP, the Wellcome Trust (U.K.) becomes a major partner; additional contributions come from Japan, France, Germany, China, and others. The project was completed in 2003. This project had the following objectives:-
- To identify all the approximately 20,000-25,000 genes in human DNA.
- To determine the sequences of the 3 billion base pairs that make up human DNA.
- Knowing locations of RFLP markers. Restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences. The principle of RFLP markers is that any genomic DNA can be differentiated according to the presence or absence of restriction enzyme sites.
- Mapping presence of genes on different chromosomes.