Multiple choice

cDNA is known to be synthesised, or manufactured from an mRNA or messenger RNA template. Which of the following statements regarding the synthesis of complementary DNA (cDNA) is incorrect?

  1. It is likely that the ssDNA forms a hairpin loop at the 3' end.

  2. DNA polymerase is added along with deoxynucleotide triphosphates.

  3. A poly-T oligonucleotide primer is hybridised onto the poly-A tail of the mature mRNA template.

  4. The mixture of mature mRNA strands is extracted from the cell.

  5. A eukaryotic cell transcribes the DNA into RNA.
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Reverse transcriptase is added along with deoxynucleotide triphosphates (A, T, G, C). This synthesises one complementary strand of DNA hybridised to the original mRNA strand.

Keep practicing — related questions