Multiple choice

Which of the following is an incorrect statement?

  1. The synthesis of DNA from mRNA involves reverse transcriptase enzyme.

  2. Both Taq polymerase and Vent polymerase are thermostable DNA polymerases.

  3. Klenov is the product of enzymatic breakdown of DNA polymerase I involving cutting of DNA strand.

  4. Alkaline phosphatase enzyme is used to remove phosphate group from the 5’ end of DNA.

  5. Ligase enzyme is used to join two DNA molecules.
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C Correct answer
Explanation

The Klenow fragment is a large protein fragment, produced when DNA polymerase - I from E. coli is enzymatically cleaved by the protease subtilisin. The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction (PCR) process. Restriction enzymes like- endonucleases are used to cut the DNA strand.

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