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Biotechnology

Covers recombinant DNA technology, genetic engineering techniques, molecular biology tools, and key scientific discoveries in biotechnology.

7 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

Identify the incorrect statement regarding recombinant DNA technology?

  1. Restriction enzymes are used to cut DNA molecule.
  2. Paul Berg’s developed the first rDNA molecule by gene splicing experiment.
  3. Gene cloning refers to the production of large number of copies of the gene.
  4. The mechanism of intake of DNA fragments from the surrounding medium by a cell is called transformation.
  5. The first successful transformation of rDNA was governed by Boyer and Cohen in 1969.
Question 2 Multiple Choice (Single Answer)

Which of the following statements is incorrect?

  1. Gray biotechnology is related to industrial process.
  2. Retrovirus is used as vector for cloning genes.
  3. Agarose is used in gel electrophoresis.
  4. Synthesis of DNA from mRNA involves transaminase enzyme.
  5. Plasmid and plasmodium are used as vector in genetic engineering.
Question 3 Multiple Choice (Single Answer)

Which of the following is an incorrect statement?

  1. DNA finger printing was developed by Alec Jaffrey.
  2. Non-coding repetitive sequences present throughout the chromosome are called as plasmids.
  3. DNA profiling technique is used in parental testing and criminal investigation.
  4. Exonucleases remove nucleotides from the terminal ends either 5’ or 3’ of DNA in one strand of duplex.
  5. EcoRI was isolated from the bacterium Escherichia coli RY13 strain.
Question 4 Multiple Choice (Single Answer)

Recombinant DNA technology in medicine is not used

  1. in the formation of insulin for diabetic patients
  2. in the formation of factor VIII for males in the treatment of Anaemia
  3. in the formation of factor IX for males and females in the treatment of haemophilia B
  4. in the formation of Granulocyte-macrophage colony-stimulating factor (GM-CSF)
  5. in the formation of tissue plasminogen activator (TPA)
Question 5 Multiple Choice (Single Answer)

Which of the following is an incorrect statement?

  1. The synthesis of DNA from mRNA involves reverse transcriptase enzyme.
  2. Both Taq polymerase and Vent polymerase are thermostable DNA polymerases.
  3. Klenov is the product of enzymatic breakdown of DNA polymerase I involving cutting of DNA strand.
  4. Alkaline phosphatase enzyme is used to remove phosphate group from the 5’ end of DNA.
  5. Ligase enzyme is used to join two DNA molecules.
Question 6 Multiple Choice (Single Answer)

Which of the following pairs of ‘scientist and corresponding discovery’ is not correctly matched?

  1. Kary Mullis - polymerised chain reaction
  2. Paul Berg - father of genetic engineering
  3. Stanley Cohen and Herbert Boyer - isolate antibiotic resistant gene
  4. Arbar, Smith and Nathan - discoveries concerning new mechanisms for the origin and dissemination of infectious diseases
  5. Willium Hays and Joshua Lederberg - plasmid
Question 7 Multiple Choice (Single Answer)

Which of the following terminologies is not correctly defined?

  1. Autoantigens: Representation of an individual’s own proteins together with other chemicals, which evidently elicit adequate immune responses.
  2. Alleles: Two or more alternative forms of a gene.
  3. Amniocentesis: pairing of complementary DNA or RNA sequences, via hydrogen bonding to form a double-stranded polynucleotide.
  4. Bt crop: Crops that contain genes for Bt toxin.
  5. Imprinting: Suppression or silencing of genes depending on the parent from which they are received.
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