Multiple choice

In humans the complementary DNA (cDNA) is, DNA synthesised from a mature mRNA template. Which of the following statements regarding the synthesis of complementary DNA (cDNA) is INCORRECT?

  1. It is likely that the ssDNA forms a hairpin loop at the 3' end.

  2. DNA polymerase is added, along with deoxynucleotide triphosphates.

  3. A poly-T oligonucleotide primer is hybridised onto the poly-A tail of the mature mRNA template.

  4. The mixture of mature mRNA strands is extracted from the cell.

  5. A eukaryotic cell transcribes the DNA into RNA.
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B Correct answer
Explanation

Reverse transcriptase is added, along with deoxynucleotide triphosphates (A, T, G, C). This synthesises one complementary strand of DNA hybridised to the original mRNA strand.

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