Multiple choice

Which among the following statements regarding the synthesis of complementary DNA (cDNA) is incorrect?

  1. A eukaryotic cell transcribes the DNA into RNA.

  2. The mixture of mature mRNA strands is extracted from the cell.

  3. A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template.

  4. DNA polymerase is added, along with deoxynucleotide triphosphates (A, T, G, C).

  5. It is likely that the ssDNA forms a hairpin loop at the 3' end.
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D Correct answer
Explanation

Reverse transcriptase is added, along with deoxynucleotide triphosphates (A, T, G, C). This synthesizes one complementary strand of DNA hybridized to the original mRNA strand.

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