Multiple choice

Which of the following is the aim of HAT (hypoxanthine-aminopterin-thymidine) selection technique?

  1. B-cell differentiation

  2. Generating antibody diversity

  3. Generating monoclonal antibodies

  4. Generating mutant species
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C Correct answer
Explanation

HAT (hypoxanthine-aminopterin-thymidine) selection technique is used for generating monoclonal antibodies. Briefly, HAT selection implies the use of HAT medium. Unfused myeloma (HGPRT negative) cells are killed by aminopterin which interferes with the conversion of tetrahydrofoliate to dihydrofoliate and prevents a series of carbon transfers. Unfused normal spleen cells die because they are not able to grow for long periods. Thus, only the fused hybridoma cells survive on this medium.

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