Hypoxanthine is also used for selecting the hybridomas based on the following:
P. Only hybridoma will grow since, it inherited the HGPRT genes from B cells and can synthesize DNA from Hypoxanthine.
Q. Myeloma cells wil not grow in culture since, de novo synthesis is blocked by aminopterin and due to lack of HGPRT enzyme.
In hybridoma technology for monoclonal Ab production antibiotic is used to select hybridoma cell over myeloma cells.
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Only P is true
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Only Q is true
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Both P and Q are true
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P is true and Q is false
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Q is true and P is false
C
Correct answer
Explanation
Both P and Q are true. In this procedure, myeoloma cells are engineered to be deficient enzyme HGPRT. After fusion of lymphocytes with HGPRT negative myeloma cells, aminopterin containing medium, supplemented with hypoxanthine and thymidine to ensure as adequate substrate supply for salvage pathway (HAT medium) is added, which kills myeloma cells but allows hybridomas to survive as they inherit HGPRT from lymphocyte parent.