Multiple choice

Which component of the HAT medium blocks the cellular biosynthesis of purines and pyrimidines from simple sugars and amino acids?

HAT medium is one of the several selective media used for the selection of hybrid cells.This medium is supplemented with hypoxanthine , aminopterin and thymidine and hence the name HAT medium. Only cell lines expressing both hypoxanthine phosphoribosyl transferase (HPRT+) and thymidine kinase (TK+) can survive in this medium.

  1. Aminopterin

  2. Hypoxanthine

  3. Thymidine

  4. Both 2 and 3

  5. Rayon acetate
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A Correct answer
Explanation

The antimetabolite aminopterin blocks the cellular biosynthesis of purines and pyrimidines from simple sugars and amino acids while HGPRT and TK supply them from hypoxanthine and thymidine.

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