Biotechnology: Immunology and Hybridoma Technology

Which of the following features are critical to hybridoma technology?

Which type of immunoglobulin is involved in hypersensitivity and allergic reactions?

Which of the following is also known as immune interferon?

Interferons (IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors

Who developed hybridoma technology?

A hybridoma is a hybrid cell obtained by fusing a B-lymphocyte with usually a tumour cell of the antibody forming system or of B-lymphocyte.The hybrid cells thus produced posses the ability to produce antibodies due to the B-lymphocyte genoome and the capacity for  indefinite growth in vitro due to the tumour (myeloma) cell involved in the fusion.Therefore, specific hybridomas are either cultured in vitro or passaged through mouse peritoneal cavity to obtain monoclonal antibodies.This is called hybridoma technology.

Which component of the HAT medium blocks the cellular biosynthesis of purines and pyrimidines from simple sugars and amino acids?

HAT medium is one of the several selective media used for the selection of hybrid cells.This medium is supplemented with hypoxanthine , aminopterin and thymidine and hence the name HAT medium. Only cell lines expressing both hypoxanthine phosphoribosyl transferase (HPRT+) and thymidine kinase (TK+) can survive in this medium.