Tag: applications of biotechnology

Quizzes (11) Questions (246)

Questions Related to applications of biotechnology

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

PCR and RFLP are employed in

  1. DNA sequencing

  2. Genetic engineering

  3. Study of enzymes

  4. Genetic transformation
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

PCR and RFLP (Restriction Fragment Length Polymorphism) are fundamental tools used in genetic engineering for DNA analysis, cloning, and mapping.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

An analysis of chromosomal DNA using Southern hybridisation technique does not use

  1. Blotting

  2. Autoradiography

  3. PCR

  4. Electrophoresis
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Southern hybridization involves DNA digestion, electrophoresis, blotting, and probe hybridization. PCR is a separate technique for amplification and is not a standard step in the Southern blotting procedure itself.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which ones are simple sequence repeats (SSRs)

  1. Minisatellite sequences

  2. Microsatellites

  3. Pseudogenes

  4. Multiple gene family.
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Simple sequence repeats (SSRs), sometimes described as genetic 'stutters,' are DNA tracts in which a short base-pair motif is repeated several to many times in tandem (e.g. CAGCAGCAG). These sequences experience frequent mutations that alter the number of repeats similar to Multigene Family, a set of genes descended by duplication and variation from some ancestral gene.

So, the correct answer is 'Multiple gene family.'

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction employs

  1. Primers and DNA ligase

  2. DNA ligase only

  3. DNA polymerase only

  4. Primers and DNA polymerase
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

PCR requires primers to define the target region and a DNA polymerase to extend the strands. DNA ligase is not used in PCR.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

PCR proceeds in three distinct steps governed by temperature. The order of the steps is 

  1. Denaturation, Synthesis, Annealing

  2. Annealing, Synthesis, Denaturation

  3. Synthesis, Annealing, Denaturation

  4. Denaturation, Annealing, Synthesis
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

The three steps of PCR are: 1) Denaturation (high heat to separate strands), 2) Annealing (cooling to allow primers to bind), and 3) Extension/Synthesis (optimal temperature for polymerase to build the new strand).

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

What is true about DNA polymerase used in PCR?

  1. It is used to ligate introduced DNA in recipient cells.

  2. It serves as selectable marker.

  3. It is isolated from a virus.

  4. It is active at high temperature.
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

The DNA polymerase used in PCR (like Taq) is derived from thermophilic bacteria, making it heat-stable and capable of remaining active through the repeated high-temperature denaturation cycles.