Tag: biotechnology: general introduction

Questions Related to biotechnology: general introduction

To confirm ELISA for AIDS which technique was used?

  1. Western blotting

  2. Northern blotting

  3. Southern blotting

  4. Eastern blotting


Correct Option: A
Explanation:

The Western blot is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract.
HIV - Human Immunodeficiency Virus
AIDS- Acquired Immuno Deficiency Syndrome.

Who launched tissue culture ?

  1. Harrison

  2. Carrel

  3. Mendel

  4. Khorana


Correct Option: A
Explanation:

Ross Granville Harrison was an American biologist and anatomist credited as the first to work successfully with artificial tissue culture.

Choose the correct statement with reference to "Dolly".

  1. She was created by taking nucleus from unfertilised egg.

  2. She was created by taking nucleus from udder cell and cytoplasm from unfertilised egg.

  3. She was created by taking cytoplasm from udder cells and nucleus from fertilised egg.

  4. She was created in the test tube.


Correct Option: B
Explanation:

To produce Dolly, sheep a cloned animal where the fin Dorset mammary cell nucleus is injected into the Scottish Blackface ewe oocyte with the help of electricity. The new fused cell is them implanted into a surrogate mother, another Scottish Blackface ewe which develops sheep a clone of fin Dorset sheep. Thus, the correct answer is option B.

Hargobind Khorana contributed to genetic engineering by synthesising 

  1. Viroid

  2. pBR 322

  3. pBR42

  4. Artificial gene


Correct Option: D

Which is the first biochemical to be produced commercially by microbial cloning and genetic engineering ?

  1. Human insulin

  2. Penicillin

  3. Interferons

  4. Fertility factors


Correct Option: A
Explanation:

Synthetic human insulin was the first biochemical product which is produced commercially by recombinant DNA technology. Before that insulin was extracted from the pig which mimics like human insulin. Currently, millions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels. Synthetic insulin is made in both bacteria and yeast.

Scaling up in biotechnology means.

  1. Development of a process in laboratory

  2. Development of a laboratory scale plant for obtaining a product

  3. Conversion of laboratory scale process to pilot plant scale

  4. Conversion of a laboratory scale plant to manufacturing unit


Correct Option: D
Explanation:

Scaling up is the process of expanding a process from small scale to a larger scale. So, here scaling up is defined as the conversion of a laboratory scale plant into a manufacturing unit.

Recombinant DNA or rDNA technology was discovered by

  1. KhoranaBateson and de Vries

  2. Sutton and Avery

  3. Stanley Cohen and Herbert Boyer

  4. Watson


Correct Option: C

The technology for production of biochips, biosensors and manipulating atoms and molecules, in order to form (build) bigger, but still microscopic, functional structures and machine is called as 

  1. Biotechnology

  2. Nanotechnology

  3. Biometallurgy

  4. Bio-mineralization


Correct Option: B
Explanation:

Nanotechnology is the science and technology of building electronic circuits and devices from single atoms and molecules. Nanotechnology is used to describe many types of research where the characteristic dimensions are less than about 1,000 nanometers. Nanotechnology deals with the molecular make-up and organization of structures. By building materials from the nano-scale up, nanotechnology is able to project the unusual material functionality found at the nanoscale to a useful, applicable measure. Nanotechnology provides new and improved methods for making almost every manufactured products - better transportation, faster computers, energy developments and medical breakthroughs.

The science of biotechnology has contributed to field of

  1. Health

  2. Pharmacy

  3. Agriculture and industry

  4. All of the above


Correct Option: D
Explanation:

Biotechnology is the branch of science which deals with the application of biological techniques for human welfare. It involves the modification of genes by the enzymatic action which leads to the production of some biological entity like antibodies, vaccines etc which benefits the mankind by improving health. The vaccines, antibodies, hormones, etc are the pharmaceutical products which help to prevent the transmission of diseases. The plants and the organisms can be genetically modified. The modified plants are resistant to the pests and diseases. The modified organisms can be useful in the production of useful products in the industry.

Thus, the correct answer is option D. 

The proper definition of biotechnology is

  1. Use of living organisms and of substances obtained from them in industry

  2. Technology used to improve species of animals

  3. Modern technology used in biological laboratories

  4. Advanced technology used in biological warfare


Correct Option: A
Explanation:
Biotechnology is the broad area of biology involving living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use" (UN Convention on Biological Diversity, Art. 2). Depending on the tools and applications, it often overlaps with the (related) fields of molecular biology, bio-engineering, biomedical engineering, biomanufacturing, molecular engineering, etc. For thousands of years, humankind has used biotechnology in agriculture, food production, and medicine. The term is largely believed to have been coined in 1919 by Hungarian engineer Károly Ereky. In the late 20th and early 21st centuries, biotechnology has expanded to include new and diverse sciences such as genomics, recombinant gene techniques, applied immunology, and development of pharmaceutical therapies and diagnostic tests. 
So the correct option is 'use of living organisms and of substances obtained from them in industry'.