Tag: biotechnology: general introduction
Questions Related to biotechnology: general introduction
Which of the following can be classified as recognition transduction component of a biosensor?
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Amplifiers
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Bioreceptors
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Transducer
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None of the above
Tissues are used as a biosensor because
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Available at low prices
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Easier to immobilise
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Cofactors for the enzyme to function are already present
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All of the above
- They are easier to immobilize.
- They maintain high stability hence not degraded by various enzymes.
- They are available at low prices.
- Process of extraction, centrifuge and purification of enzymes can be avoided.
- They are necessary co-factors.
Which of the following are squares of glass, smallest than the smallest postage stamp
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Biochip
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Proteomics
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Forensic technologies
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None
Biochips are squares of glass, smaller than smallest postage stamp, covered with millions of DNA strand. Biochip is a tool allowing rapid screening of gene profile to detect genes. They are called gene microarrays. A biochip contains all known human genes.
Is a tool allowing rapid screening of gene profile to detect genes.
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Biochip
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Proteomics
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Forensic technologies
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None
Biochips are squares of glass, smaller than smallest postage stamp, covered with millions of DNA strand.
Arrange the following parts of a biosensor in chronological order.
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A) Bioreceptors, Transducer, Amplifier, Microelectronics
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B) Bioreceptors, Amplifier, Microelectronics, Transducer
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C) Transducer, Bioreceptors, Amplifier, Microelectronics
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D) Bioreceptors, Amplifier, Microelectronics, Transducer
The type of bio transducer used in the biosensors are ___________.
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Electrochemical biosensors
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Piezoelectric biosensors
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Pyroelectric biosensors
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All of the above
The type of transducer used in the sensors are
The amperometric transducers used in biosensors is used to detect _________________.
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Change in current during electrochemical oxidation or reduction
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Change in conductive properties of the sample solution
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Change in potential proportional to the concentration of analyte
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None of the above
Amperometric transducers detect change in current as a result of electrochemical oxidation or reduction.
The commercial blood glucose biosensor uses which of the following enzymes?
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Glucokinase
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Glucose oxidase
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Phosphofructokinase
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Glycosidases
Glucose oxidase is widely used for the determination of free glucose in body fluids and in the food industry. It also has many applications and typically enzyme assays for biochemistry including biosensors.
The biological content which acts as a sensor in biosensor can be __________.
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Nucleic acid
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Plant proteins
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Lectins
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All of the above
- The biological component of biosensor can be divided into two distinct groups, i.e., catalytic and non-catalytic.
- The catalytic group includes enzymes, microorganisms and tissues, while the non-catalytic consists of antibodies, receptors, nucleic acids and some proteins such as lectins etc.
- The recognition process is based on the principle of complementary base pairing adenine:thymine and cytosine:guanine in DNA.
- If the target nucleic acid sequence is known,complementary sequences can be synthesized, labelled, and then immobilized on the sensor.
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The most important applications of lectin-based biosensors are in the field of medicine and therapeutics.
Why are enzymes considered as a good bioreceptors for biosensors?
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They have specific binding capabilities
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They have good catalytic power
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They can detect analytes
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All of the above
Bioreceptor interacts with the specific analyte of interest to produce an effect which is measured by the transducer. The main reasons for the common use of enzymes in biosensors are:
- Ability to catalyze a large number of reactions;
- Potential to detect a group of analytes such as substrates, products, inhibitors, and modulators of the catalytic activity and
- Suitability with several different transduction methods for detecting the analyte