Tag: biotechnology and genetic engineering

Questions Related to biotechnology and genetic engineering

Nitrocellulose and nylon are used in which of the following technique of immobilisation?

  1. Entrapment

  2. Encapsulation

  3. Adsorption

  4. Cross-linking


Correct Option: B

The carriers or supporters which are used for covalent bonding for immobilisation of enzymes?

  1. Cellulose

  2. Collagen

  3. Gelatin

  4. All of the above


Correct Option: D

The pore size of the matrix used for immobilisation of enzymes depends upon

  1. Concentration of enzyme

  2. Molecular weight of enzyme

  3. Catalytic nature of enzyme

  4. Both A and B


Correct Option: D

Which of the following is a method of adsorption used for immobilisation of enzymes?

  1. Reactor loading process

  2. Electrode position process

  3. Diazoation

  4. Both A and B


Correct Option: D

Which of the following can be used as matrixes for entrapment?

  1. Agar

  2. Gelatin

  3. Cellulose triacetate

  4. All of the above


Correct Option: D

Which of the following is a common use of somatic hybridization?

  1. Transfer of jumping genes

  2. Transfer of some of the chromosomes between divergent species

  3. Transfer of resistant gene

  4. None of the above


Correct Option: B
Explanation:

The process by which protoplasts of two different plant species are fused together and transfer of some of the chromosomes occurs between two species to form hybrids is called as somatic hybridization and the hybrids that are produced is known as somatic hybrids. The steps of somatic hybridization involve - isolation of protoplasts, the fusion of different protoplasts and selection of hybrids. 

Thus, the correct answer is option B.

Match List-I with List-II and select the correct answer.

List-I List-II
a. Ficus benghalensis $1$. Fibre
b. Melia azadirachta $2$. Pesticide
c. Cocos nucifera $3$. Latex
d. Hevea brasiliensis
  1. A-$1$, B-$2$, C-$3$, D-$1$

  2. A-$1$, B-$1$, C-$2$, D-$3$

  3. A-$3$, B-$3$, C-$1$, D-$2$

  4. A-$3$, B-$2$, C-$1$, D-$3$


Correct Option: A

Yeast cells can be immobilised in

  1. Silica gel

  2. Calcium alginate

  3. Porcelain column

  4. Both A and B


Correct Option: B

The top five crops, as on date, in terms of annual world production in millions of metric tons belong to the families ________________.

  1. Gramineae and Solanaceae

  2. Gramineae and Cruciferae

  3. Gramineae and Leguminasae

  4. Leguminosae and Malvaceae


Correct Option: A

Which of the following is a method of covalent bonding used during immobilisation?

  1. Diazotation

  2. Phosphorylation

  3. Dehydrogenation

  4. None of the above


Correct Option: A
Explanation:
Covalent bonding is one of the commonly used methods of enzyme immobilization which engages the formation of covalent bonds between the enzymes & the support. Diazotation is a method of convalent bonding which displays the bonding between the amino group of support & tyrosyl or histidyl group of enzyme. 
So, the correct answer is 'Diazotation'