Tag: biotechnology and genetic engineering

Questions Related to biotechnology and genetic engineering

Which one of the following is used in industrial preparation of ethanol?

  1. Lactobacillus

  2. Azotobacter

  3. Penicillium

  4. Saccharomyces


Correct Option: D
Explanation:
Yeast are simple unicellular organisms belonging to the Kingdom Fungi. The alcoholic fermentation is conducted by yeast of the genus Saccharomyces. The two common species involved are S. cerevisiae and S. bayanus. Saccharomyces converts the glucose, fructose and sucrose found in grape must and juice into ethanol via the process of fermentation. In fermentation, an organic compound, in this case acetaldehyde, serves as terminal electron acceptor. This leads to the production of ethanol.

Vitamin ${B} _{12}$ is formed during fermentation of

  1. Ashbya gossypii

  2. Rhizopus stolonifer

  3. Propionibacteria

  4. Saccharomyces cerevisiae


Correct Option: C
Explanation:
Vitamin B$ _{12}$ is one of the important vitamins of B complex group. It is also known as cobalamin or cobamide. 
Direct fermentation employing Bacillus megaterium, Propionibacterium freudenreichii, Propionibacterium shermanii and Pseudomonas is used for commercial production of vitamin B$ _{12}$. 

Citric acid is produced by

  1. Aspergillus niger

  2. Streptococcus lactis

  3. Acetobacter syboxydans

  4. Candida utilis


Correct Option: A
Explanation:

Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus. Various strains of A. niger are used in the industrial preparation of citric acid and gluconic acid.

Saccharomyces cerevisiae is used in the formation of

  1. Ethanol

  2. Methanol

  3. Acetic acid

  4. Antibiotics


Correct Option: A
Explanation:

S. cerevisiae is known for its role in food production. It is the critical component in the fermentation process that converts sugar into ethyl alcohol (ethanol), an ingredient shared in beer, wine and distilled beverages. It is also used in the baking process as a leavening agent; yeast releasing gas into their environment results in the spongy-like texture of breads and cakes. 

Which of the following is used to manufacture ethanol from starch?

  1. Penicillin

  2. Saccharomyces

  3. Azotobactor

  4. Lactobacillus


Correct Option: B
Explanation:

S. cerevisiae is known for its role in food production. It is the critical component in the fermentation process that converts sugar into ethyl alcohol (ethanol), an ingredient shared in beer, wine and distilled beverages. It is also used in the baking process as a leavening agent; yeast releasing gas into their environment results in the spongy-like texture of breads and cakes. 

During the formation of bread, it becomes porous due to release of CO$ _{2}$ by the action of

  1. Yeast

  2. Bacteria

  3. Virus

  4. Protozoan


Correct Option: A
Explanation:

In a bread dough, where the oxygen supply is limited, the yeast can only partially breakdown the sugar. Alcohol and carbon dioxide are produced in this process known as alcoholic fermentation. The carbon dioxide produced in these reactions causes the dough to rise, and the alcohol produced mostly evaporates from the dough during the baking process. During fermentation, each yeast cell forms a centre around which carbon dioxide bubbles form. Thousands of tiny bubbles, each surrounded by a thin film of gluten form cells inside the dough piece. The increase in dough size occurs as these cells fill with gas.

Thus, the correct answer is option A.

Which one is not used in the production of yogurt?

  1. Streptococcus lactis

  2. Streptococcus thermophilous

  3. Lactobacillus bulgaris

  4. Acetobacter aceti


Correct Option: D
Explanation:

Yogurt is a food produced by bacterial fermentation of milk. The bacteria used to make yogurt are known as yogurt cultures. Fermentation of lactose by these bacteria produces lactic acid, which acts on milk protein to give yogurt its texture and its characteristic tang. Some representative strains of yogurt cultures are Streptococcus lactis, S. cremoris, S. thermophilus, Lactobacillus bulgaricus, L. acidophilus, and L. plantarum.

Vitamin B$ _{12}$ is produced by

  1. Propionibacterium

  2. Ashbya gossypii

  3. Saccharomyces

  4. Rhizopus


Correct Option: A
Explanation:

Vitamin B$ _{12}$ cannot be made by plants or animals as only bacteria have the enzymes required for its synthesis. Members of the genus Propionibacterium are widely used in the production of vitamin B$ _{12}$.

Thus, the correct answer is option A.

Which one of the following is used in the making of bread?

  1. Rhizopus stolonifer

  2. Saccharomyces cerevisiae

  3. Zygasaccharomyces

  4. Saccharomyces ludwigii


Correct Option: B
Explanation:

Baker's yeast is the common name for the strains of yeast commonly used as a leavening agent in baking bread and bakery products, where it converts the fermentable sugars present in the dough into carbon dioxide and ethanol. Baker's yeast is of the species Saccharomyces cerevisiae, which is the same species (but a different strain) commonly used in alcoholic fermentation, which is called as brewer's yeast.

The new strain of bacteria produced by biotechnology in alcohol industry is

  1. Escherichia coli

  2. Saccharomyces cerevisiae

  3. Bacillus subtilis

  4. Pseudomonas putida


Correct Option: D
Explanation:

Microorganisms isolated based on their ability to degrade hydrocarbons often belong to the genus Pseudosmonas. These bacteria have several catabolic pathways and are easily handled and grown in the laboratory. Pseudomonas putida, a Gram-negative proteobacterium, possesses the outstanding ability to tolerate high concentrations of different hydrocarbons.