Tag: biotechnology

Questions Related to biotechnology

Lactic acid bacteria (LAB) at suitable temperature converts milk to curd, which improves its nutritional quality by enhancing vitamin

  1. A

  2. B

  3. C

  4. D


Correct Option: B
Explanation:

Dairy products have generally been considered an excellent source of high-quality protein, calcium, potassium, phosphorus, magnesium, zinc, and the B vitamins riboflavin, niacin, vitamin B-6, and vitamin B-12. The final nutritional composition of yogurt is affected by the species and strains of bacteria used in the fermentation, the source and type of milk solids that may be added before fermentation, and the temperature and duration of the fermentation process.

In olden days, cheese was prepared by

  1. Aspergillus

  2. Rennet enzyme

  3. Clostridium bacteria

  4. None of the above


Correct Option: B
Explanation:

Traditional animal rennet is an enzyme derived from the stomachs of calves, lambs or goats. Rennet is used in the production of most cheeses. Chymosin, its key component, is a protease enzyme that curdles the casein in milk. 

In the formation of ascorbic acid, the micro-organism used is

  1. Acetobacter

  2. Streptomyces sp.

  3. Bacillus megatherium

  4. Propioni bacterium


Correct Option: A
Explanation:

Acetobacter suboxydans is a gram-negative bacteria of the order rhodospirilla. It is used in the formation of ascorbic acid. A. suboxydans is widely used commercially in the production of ketonic acids like ascorbic acid (Vitamin C). 

In the production of leavened bread, the following is used

  1. Bacterium

  2. Yeast

  3. Rhizopus

  4. None of the above

  5. Both (A) and (B)


Correct Option: E
Explanation:

Sourdough (leavened bread) is a bread product made by a long fermentation of dough using naturally occurring Lactobacilli and yeasts. The most commonly used yeast species in the production of sourdough are Kazanchastania exigua (Saccharomyces exiguous), Saccharomyces cerevisiae, Candida Milleri, and Candida humilis

Germinating barley seeds are employed in the preparation of

  1. Cheese

  2. Wine

  3. Beer

  4. Lactic acid


Correct Option: C
Explanation:

Malted barley is barley that has been allowed to germinate (sprout) to a degree and is then dried. The germination process converts starch, the seed's stored energy, into simpler sugars used in its initial growing stage. The conversion of starch to sugar is accomplished by diastase enzymes that the seed produces during this process. The germination and drying stages capture fermentable sugars, soluble starch, and the diastase enzymes for beer brewing. 

Which of the following organic acids was produced by fermentation?

  1. Oxalic acid

  2. Lactic acid

  3. Citric acid

  4. Propionic acid


Correct Option: B
Explanation:

Lactic acid fermentation is a biological process by which glucose and other six-carbon sugars are converted into cellular energy and the metabolite lactate. Lactic acid fermentation is caused by some fungi and bacteria. The most important lactic acid producing bacteria is Lactobacillus. The presence of lactic acid, produced during the lactic acid fermentation is responsible for the sour taste and for the improved microbiological stability and safety of the food. This lactic acid fermentation is responsible for the sour taste of dairy products such as cheese, yoghurt 

Rennet is used in

  1. Fermentation

  2. Cheese making

  3. Bread making

  4. Synthesis of antibiotic


Correct Option: B
Explanation:

Rennet is a complex of enzymes produced in stomachs of ruminant mammals which is used in the production of most cheeses. Chymosin, its key component, is a protease enzyme that curdles the casein in milk, helping young mammals digest their mothers' milk. It can also be used to separate milk into solid curds used for cheesemaking and liquid whey.

Enzymes can be immobilized by

  1. Cross-linking enzyme molecules

  2. Covalently attaching to a solid support

  3. Entrapping them in gel

  4. All of the above


Correct Option: D
Explanation:

There are four principal methods available for immobilizing enzymes as follows:

(a) Enzyme non-covalently adsorbed to an insoluble particle; 
(b) Enzyme covalently attached to an insoluble particle; 
(c) Enzyme entrapped within an insoluble particle by a cross-linked polymer; 
(d) Enzyme confined within a semi-permeable membrane.

The process in which the yeast cells perform is called as 

  1. Transpiration

  2. Pastuerization

  3. Fermentation

  4. Effervescence


Correct Option: C
Explanation:

Fermentation is an anaerobic process in which energy can be released from glucose even though oxygen is not available. Fermentation occurs in yeast cells. Yeasts are able to participate in fermentation because they have the necessary enzyme to convert pyruvic acid to ethyl alcohol. 

Citric acid is produced by

  1. Aspergillus niger

  2. Streptococcus lactic

  3. Acetobacter suboxydans

  4. Candida utilis


Correct Option: A
Explanation:

Large-scale, commercial production of citric acid is done by the microorganism, Aspergillus niger by aerobic fermentation of sucrose in beet molasses, a mould belonging to the same family as the penicillins.