Tag: biotechnology

Questions Related to biotechnology

Which of the following is used in the production of leavened bread?

  1. Aspergillus

  2. Rhizopus

  3. Saccharomyces

  4. None of the above


Correct Option: C
Explanation:

The selected strains of Saccharomyces cerevisiae, used in baking industry to bring about desired changes in flavour and texture of the baked products, are sold as baker's yeast. These yeasts have ability to ferment the sugar in the dough. The carbon dioxide produced during fermentation is responsible for the leavening (i,e., raising of the dough). Both CO$ _2$ and alcohol escape during baking so that the bread and other baked products become porous and soft.

Thus, the correct answer is option C.

The microorganism useful for fermentation of industrial production of beer is

  1. Streptomyces olivaceous

  2. Aspergillus niger

  3. Penicillium notatum

  4. Saccharomyces cerevisiae


Correct Option: D
Explanation:

Yeasts are the main producers of wine and beer. In that the most important and high yielding strain is the saccharomyces cerevisiae. They ferment the sugars that comes the grapes and barley. 

Streptomyces olivaceous is used in the production of antibiotics. 
Aspergillus niger is used in the production of organic acids like the citric acid 
Pencillium notatum is used in the production of penicillin.
Hence, the correct answer is option D.


The utility of fungi for steroid conversion was demonstrated by

  1. Kohler and Milstein

  2. Murray and Peterson

  3. Kornberg and Miller

  4. Waksman and Monad


Correct Option: B
Explanation:

Murray and Peterson demonstrated the utility of fungi for steroid conversion by using the Rhizopus stolonifer. Whereas the technique for development of monoclonal antibody was demonstrated by Kohler and Milstein. Waksman and Monad demonstrate the synthesis of streptomycin antibody from streptomyces species. Kornberg and Miller are known for the purification of the DNA polymerase 1 enzyme. Thus, the correct answer is option B.

Aspergillus niger is commercially used to prepare

  1. Lactic acid

  2. Acetic acid

  3. Citric acid

  4. Methanoic acid


Correct Option: C
Explanation:

Aspergillus is utilized industrially in a number of ways. Most sodas and soft drinks contain citric acid as the main ingredient. Citric acid is also used in other drinks, many candies, canned goods, baked goods, etc. It is too expensive to isolate the citric acid from citrus fruits so it is produced in the large-scale fermentation of sugar by utilizing Aspergillus niger

Cider is prepared by

  1. Action of Lactobacillus on milk.

  2. Action of Saccharomyces on apple juice.

  3. Action of Saccharomyces on cane sugar.

  4. Action of Bacillus on ramie.


Correct Option: B
Explanation:

Cider is also called the apple cider. This is an alcoholic product that is prepared from the fermentation of apples with the yeast Saccharomyces. Whereas Lactobacillus in milk converts it into the curd, Saccharomyces produces alcohol by the fermentation process in cane sugar and Bacillus Is used to bleach ramie fiber. Thus, the correct answer is option B.

Saccharomyces cerevisiae is used in the formation of

  1. Ethanol

  2. Methanol

  3. Acetic acid

  4. Antibiotics


Correct Option: A
Explanation:

The term "Yeast" is a morphological term that refers to a one-celled fungus. Most yeasts, including Saccharomyces reproduce by budding, where the daughter cells bleb off from a small pore in the side of the mother cell. S. cerevisiae is famously known for its role in food production. It is the critical component in the fermentation process that converts sugar into alcohol, an ingredient shared in beer, wine and distilled beverages. It is also used in the baking process as a leavening agent; yeast releasing gas into their environment results in the spongy-like texture of breads and cakes. Because of its role in fermentation, humans have known about and used Saccharomyces cerevisiae for a long time. In a process called as fermentation, yeast feeds off sugars from their substrate and convert it to ethanol, giving these beverages their alcoholic content. Depending on the beverage, yeast is incorporated into the creation process in several ways.

An enzyme used in cheese making is

  1. Protease

  2. Rennet

  3. Glucoamylase

  4. Lactase


Correct Option: B
Explanation:
Animal rennet (bovine chymosin) is conventionally used as a milk-clotting agent in dairy industry for the manufacture of quality cheeses with good flavour and texture. Owing to an increase in demand for cheese production world wide, i.e., 4% per annum over the past 20 years, approximating 13.533 million tons – coupled with reduced supply of calf rennet, has therefore led to a search for rennet substitutes, such as microbial rennets. At present, microbial rennet is used for one-third of all the cheese produced world wide.
Thus, the correct answer is option B.

Yeast is used in the formation of 

  1. Ammonia

  2. Alcohol

  3. Curd

  4. Petrol


Correct Option: B
Explanation:

Ethanol fermentation is a biological process to produce ethanol and carbon dioxide as metabolic waste products. Because yeasts perform this conversion in the absence of oxygen, alcoholic fermentation is considered an anaerobic process. 

Thus, the correct answer is option B.

Which one of the following is used in the manufacture of alcohol?

  1. Bacteria

  2. Water molds

  3. Yeasts

  4. Slime molds


Correct Option: C
Explanation:
The overall process of fermentation is to convert sugar glucose (C$ _6$H$ _{12}$O$ _6$) to an alcohol (CH$ _3$CH$ _2$OH) and carbon dioxide gas (CO$ _2$). The reactions within the yeast to make this happen are very complex but the overall process is as follows: C$ _6$H$ _{12}$O$ _6$ ====> 2(CH$ _3$CH$ _2$OH) + 2(CO$ _2$)
 Sugar ====> Alcohol + Carbon dioxide gas. 
The most important type of fermentation is alcoholic fermentation, in which the action of zymase secreted by yeast converts simple sugars, such as glucose and fructose, into ethyl alcohol and carbon dioxide.

Saccharomyces is commonly used in the production of

  1. Ethyl alcohol

  2. Curd

  3. Citric acid

  4. Acetic acid


Correct Option: A
Explanation:

Saccharomyces cerevisiae (Common name is Baker's or brewer's yeast) is a species of yeast generally used in the production of ethyl alcohol in beer and wine making process. In the production of ethyl alcohol, the fungus Saccharomyces produces the enzyme zymase. This zymase will then acts on Glucose to produce ethyl alcohol. Thus, the correct answer is option A.