Multiple choice

Why is an RNA primer necessary in DNA replication?

  1. Provides a 3' end that DNA polymerase can add nucleotides to

  2. To support the unwinding of DNA by DNA gyrase

  3. To support the unwinding of DNA by Helicase

  4. Provides a 5' end that DNA polymerase can add nucleotides to
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A Correct answer
Explanation

DNA polymerase cannot initiate synthesis on a bare template strand; it requires an existing 3' end to attach new nucleotides. The RNA primer provides this necessary 3' hydroxyl group.

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