Multiple choice

Which among the following statements regarding the differential extraction of DNA is incorrect?

  1. This method is used in the extraction of DNA from vaginal epithelial cells.

  2. Differential extraction disrupt the peptide bonds present in the coating of the sperm cell.

  3. Through this process, the DNA from two different types of cells can be extracted without mixing their contents.

  4. The first part of the differential lysis is carried out under mild conditions to gain the female DNA.

  5. Due to the high yield of DNA, the chance of a successful DNA-profile by downstream analysis is significantly increased.
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B Correct answer
Explanation

Differential extraction uses a chemical called dithiothreitol (DTT) to disrupt the sulfur bonds in the coating of the sperm cell in order to extract its DNA.

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