Multiple choice

Which of the following DNA profiling techniques is employed where a differential extraction of DNA is not possible?

  1. RFLP analysis (Restriction fragment length polymorphism)

  2. PCR analysis (polymerase chain reaction)

  3. STR analysis (Short tandem repeats)

  4. AmpFLP (Amplified fragment length polymorphism)

  5. Y-chromosome analysis
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E Correct answer
Explanation

Recent innovations have included the creation of primers targeting polymorphic regions on the Y-chromosome (Y-STR), which allows resolution of a mixed DNA sample from a male and female or cases in which a differential extraction is not possible. Y-chromosomes are paternally inherited, so Y-STR analysis can help in the identification of paternally related males.

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